Apocynin Dietary Supplementation Delays Mouse Ovarian Ageing

Table 1

List of primers used in RT-PCR reactions.

Primers		Sequence	Annealing temperature	Fragment (bp)
Col1α1	Fwd	5-GACGCATGGCCAAGAAGACA-3	60°C	85
	Rev	5-CTCGGGTTTCCACGTCTCAC-3
Col3α1	Fwd	5-AGCTTTGTGCAAAGTGGAACC-3	58°C	114
	Rev	5-ATAGGACTGACCAAGGTGGC-3
Col5α1	Fwd	5-CCTGGTTCAGTGAATTCAAGCG-3	60°C	81
	Rev	5-TCATTTGTACCACGCCCACG-3
TGF-β1	Fwd	5-ATTCCTGGCGTTACCTTGG-3	60°C	120
	Rev	5-AGCCCTGTATTCCGTCTCCT-3
TNF-α	Fwd	5-CCCTCACACTCAGATCATCTTCT-3	60°C	61
	Rev	5-GCTACGACGTGGGCTACAG-3
IL-1β	Fwd	5-TGACGGACCCCAAAAGATGA-3	60°C	87
	Rev	5-TGCTGCGAGATTTGAAGCTG-3
CCL5	Fwd	5-TGCCCACGTCAAGGAGTATT-3	60°C	84
	Rev	5-ACTTGGCGGTTCCTTCGAG-3
MMP2	Fwd	5-TGTCGCCCCTAAAACAGACA-3	58°C	65
	Rev	5-TGGGGCAGCCATAGAAAGTG-3
MMP9	Fwd	5-CCTGGAACTCACACGACATCT-3	62°C	72
	Rev	5-CACGCCAGAAGAATTTGCCAT-3
MMP12	Fwd	5-GGGCTGCTCCCATGAATGAC-3	56°C	85
	Rev	5-GTCATTGGAATTCTGTCCTTTCCA-3
TIMP1	Fwd	5-GGTGTGCACAGTGTTTCCCTGTTT-3	60°C	72
	Rev	5-TCCGTCCACAAACAGTGAGTGTCA-3
TIMP2	Fwd	5-GGATTCAGTATGAGATCAAGC-3	55°C	145
	Rev	5-GCCTTTCCTGCAATTAGATAC-3
18S	Fwd	5-CGCCGCTAGAGGTGAAATTC-3	60°C	67
	Rev	5-CATTCTTGGCAAATGCTTTCG-3

Fwd: forward; Rev: reverse; bp: base pairs.