Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Figure 4

TEM micrographs of human sperm treated with H₂O₂ without the C. sativa leaf extract (a) and H₂O₂ with the C. sativa leaf extract diluted to 1 : 200 (b). In (a), a spermatozoon with the reacted acrosome (arrow) and altered chromatin texture, particularly evident along the edge of the cell, is shown. The membrane is broken. In (b), a normal spermatozoon with integer structures is shown. Bars: 1 μM.

(a)

(b)