Nickel Enhances Zinc-Induced Neuronal Cell Death by Priming the Endoplasmic Reticulum Stress Response

Figure 5

GT1-7 cells (6-well culture plates at a density of cells per well) were pretreated with the indicated concentrations (mM) of carnosine just before Ni²⁺/Zn²⁺ treatment. Next, GT1-7 cells were incubated in the absence (control) or presence of NiCl₂ (Ni, 40 μM) and ZnCl₂ (Zn, 25 μM) for 4 h. Total RNA was extracted and subjected to real-time RT-PCR using primer sets specific for each gene. Values were normalized to Gapdh and expressed relative to the control. Values represent values are described in the figure when (black: vs. control, red: vs. Zn(25)/Ni(40)).

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