Irisin Ameliorates Intervertebral Disc Degeneration by Activating LATS/YAP/CTGF Signaling

Figure 3

Irisin treatment restored the metabolism of the ECM in TNF-α-induced NPCs. (a) CCK-8 assay was performed to detect the effects of irisin on the cells’ viability at various concentrations (0, 25, 50, 100, 200, and 400 ng/ml) at 24 and 48 hours. (b) The relative expression levels of different genes (COL2A1, MMP9, ADAMTS4, and ADAMTS5) were detected using qPCR in different groups. (c) The protein expression levels of COL2A1, MMP9, MMP13, ADAMTS4, and ADAMTS5 were detected by western blot in different groups. (d) The expression levels of COL2A1 and ADAMTS4 were detected by immunofluorescence in different groups. DAPI was used to stain for the nuclei. Images (magnification: ×400, scale bar: 50 μm). In (b)–(d), irisin was used at the concentration of 100 ng/ml and TNF-α was used at the concentration of 10 ng/ml. , .

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