P-Glycoprotein Exacerbates Brain Injury Following Experimental Cerebral Ischemia by Promoting Proinflammatory Microglia Activation

Figure 1

P-glycoprotein influences ischemic infarction and neurological deficits in experimental ischemic stroke. Mice were intracerebroventricularly injected with P-glycoprotein (P-gp) siRNA or negative control (NC) siRNA (1.5 μL/10 g body weight), P-gp p-AAV or NC p-AAV (2.5 μL/10 g body weight), 48 hr or 14 days prior to MCAO/R surgery. Twenty-four hours after the surgery, mice were subjected to neurological behavior testing, and brains were harvested for western blot or TTC analyses. (a, b) Western-blotting quantification of P-gp levels in brain cortex (n = 4). (c, d) Representative TTC staining images and quantification of infarct volume (n = 6). (e) Neurological behavior assessed by Bederson score (n = 6). One-way ANOVA followed by the post hoc least significant difference test or Games Howell test for (a), (b), and (d). Mann–Whitney test for (e). All data are mean ± SD; , between two groups.

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