P-Glycoprotein Exacerbates Brain Injury Following Experimental Cerebral Ischemia by Promoting Proinflammatory Microglia Activation

Figure 3

P-glycoprotein modulates microglial polarization in experimental ischemic stroke. Mice were intracerebroventricularly injected with P-glycoprotein (P-gp) siRNA or negative control (NC) siRNA (1.5 μL/10 g body weight), P-gp p-AAV or NC p-AAV (2.5 μL/10 g body weight), 48 hr or 7 days prior to MCAO/R surgery. Twenty-four hours after the surgery, brains were harvested for immunofluorescence assay. (a) Immunofluorescence colocalization of microglial polarization status markers (CD16, iNOS, CD206, and Arg-1) in the microglia (Iba1) (n = 4). Scale bars, 100 μm. (b) Quantification of CD16, iNOS, CD206, and Arg-1 localization in the microglia. (c) Coronal brain diagrams showing locations of regions for immunofluorescence staining analysis in infarct cortex. One-way ANOVA followed by the post hoc least significant difference test. All data are mean ± SD; between two groups.

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