P-Glycoprotein Exacerbates Brain Injury Following Experimental Cerebral Ischemia by Promoting Proinflammatory Microglia Activation

Figure 7

P-glycoprotein silence or overexpress in endothelial cells alters microglial polarization following oxygen glucose deprivation/reoxygenation. Endothelial cells (bEnd.3) were transfected with P-glycoprotein (P-gp) or negative control (NC) siRNA, P-gp or NC pcDNA3.1 plasmid, or untransfected, and then subjected to either oxygen glucose deprivation/reoxygenation (OGD/R) treatment or normal culture conditions. Following 24 hr coculture with microglia (BV2), BV2 cells were harvested for immunofluorescence assay. (a) Representative immunofluorescence staining images and quantification of microglial polarization status markers (CD16, iNOS, CD206, and Arg-1) expressions (n = 3). (b) Quantification of CD16, iNOS, CD206, and Arg-1 expressions (n = 3). Scale bars, 100 μm. One-way ANOVA followed by the post hoc least significant difference test. All data are mean ± SD; between two groups.

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