P-Glycoprotein Exacerbates Brain Injury Following Experimental Cerebral Ischemia by Promoting Proinflammatory Microglia Activation

Figure 8

P-glycoprotein silence or overexpress in endothelial cells regulates GR nuclear translocation and further GMD activation following oxygen glucose deprivation/reoxygenation. Endothelial cells (bEnd.3) were transfected with P-glycoprotein (P-gp) or negative control (NC) siRNA, P-gp or NC pcDNA3.1 plasmid, or untransfected, and then subjected to either oxygen glucose deprivation/reoxygenation (OGD/R) treatment or normal culture conditions. BEnd.3 cells were harvested for immunofluorescence and western-blotting assays. (a, b) Representative immunofluorescence staining images and quantification of nuclear and cytoplasmic localization (ratio of nuclear to cytoplasmic location) of GR (n = 3). (c–e) Representative western-blotting images and quantifications of cytoplasm GR and nucleus GR expressions (n = 3). (f, g) Representative western-blotting images and quantifications of DCP1A, UPF1, and PNRC2 expressions (n = 3). Scale bars, 100 μm. One-way ANOVA followed by the post hoc least significant difference tests. All data are mean ± SD; between two groups.

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