Abatacept: A Promising Repurposed Solution for Myocardial Infarction-Induced Inflammation in Rat Models

Figure 3

Effect of Abatacept on histopathological changes, apoptosis pathway protein expression and immunohistochemical analysis in ISO-induced rat model of myocardial necrosis. (a) Histopathology (H&E): (n = 3, 20x scale bar = 100 µm). Histopathological grading for necrosis, edema, and inflammation: (+++) severe; (++) moderate; (+) mild; (−) no. C (F); (b) apoptosis pathway protein (Bax, Bcl-2, and Caspase-3) expression: data are expressed as mean ± SEM.; n = 3 in each group. (c) Effect of Abatacept on immunohistochemical analysis of bax (IA–E), Bcl-2 (IIA–E), and Caspase-3 (IIIA–E); (A) normal; (B) ISO-C; (C) ABT2.5 + ISO; (D) ABT5 + ISO; (E) ABT10 + ISO; (n = 3). , , and . ISO-C, isoproterenol control; ABT2.5+ISO, Abatacept 2.5 mg/kg + isoproterenol; ABT5 + ISO, Abatacept 5 mg/kg + isoproterenol; ABT10+ISO, Abatacept 10 mg/kg + isoproterenol.

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