ACA-28, an ERK MAPK Signaling Modulator, Exerts Anticancer Activity through ROS Induction in Melanoma and Pancreatic Cancer Cells

Figure 1

ACA-28 induces the expression of genes associated with Nrf2. (a) Workflow for the identification of genetic pathways altered by ACA-28. (b) Bar graph showing the number of Transcript Cluster IDs (TC IDs) upregulated (>1.5 fold) and downregulated (<−1.5 fold) by ACA-28, among the 67528 IDs identified by human transcriptome array analysis. (c) Bubble plot representing the top five canonical pathways enriched by ACA-28, identified by Ingenuity pathway analysis. Bubble size, the number of genes assigned to a pathway; color, the overlapping percentage for genes belonging to the indicated pathways. (d) Scatter plot showing the expression level of TC IDs with annotated gene names. The dots indicate the intensity levels of each TC ID. Representative TC IDs corresponding to the top 11 genes upregulated by ACA-28 are shown in red. The 11 genes contain consensus binding motifs for Nrf2, as shown in Figure S1.

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