ACA-28, an ERK MAPK Signaling Modulator, Exerts Anticancer Activity through ROS Induction in Melanoma and Pancreatic Cancer Cells

Figure 5

A549, a cell line with constitutively activated Nrf2, is resistant to ACA-28. (a) A representative western blot showing the expression of Nrf2 and GAPDH in SK-MEL-28 and A549 cells. (b) Relative quantification of the Nrf2 protein levels shown in (a). GAPDH was used as an internal control. Data are expressed as the mean ± standard deviation (SD) from three independent experiments. (c) Comparison of the viability of SK-MEL-28 and A549 cells after exposure to the indicated concentrations of ACA-28 for 48 hr. Cell viability was assessed with the WST-8 assay. Data are expressed as the relative percentage to that of DMSO treatment. Bars represent the mean ± standard deviation (SD) from two independent experiments (n.s.: not significant, , , Williams’ post hoc test compared to the respective DMSO control) (^#, ^##, unpaired Student’s t-test between two groups).

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