ACA-28, an ERK MAPK Signaling Modulator, Exerts Anticancer Activity through ROS Induction in Melanoma and Pancreatic Cancer Cells

Figure 6

Manipulation of Nrf2 protein levels affects the ACA-28 sensitivity in HeLa cells. (a) HeLa cells were transfected with pcDNA5-Nrf2-HA or the control vector and subsequently treated with the indicated concentrations of ACA-28 for 24 hr. (b) HeLa cells were transfected with scrambled siRNA or Nrf2 siRNA and subsequently treated with the indicated concentrations of ACA-28 for 24 hr. Cell viability was assessed with the WST-8 assay. Data are expressed as the relative percentage to that of nontransfected cells treated with DMSO (0 µM of ACA-28). Bars represent the mean ± SD of four independent experiments (n.s.: not significant, , , Williams’ post hoc test compared to the respective DMSO control) (^#, ^##, unpaired Student’s t-test between two groups).

(a)

(b)