Adaptive Redox Response of Mesenchymal Stromal Cells to Stimulation with Lipopolysaccharide Inflammagen: Mechanisms of Remodeling of Tissue Barriers in Sepsis
Table 1
qRT-PCR assessment of gene transcription in MSCs challenged with LPS.
LPS
IL1A
IL1β
IL6
IL8
iNOS
ng/mL
Expression, a.u.
Expression, a.u.
Expression, a.u.
Expression, a.u.
Expression, a.u.
0
1 ± 3.25
1 ± 1.82
1 ± 1.54
1 ± 1.41
1 ± 3.14
50
194 ± 2.77*
317 ± 1.63*
99 ± 1.48*
26 ± 1.8*
882 ± 2.46*
100
265 ± 2.33*
465 ± 1.78*
155 ± 1.4*
46 ± 1.36*
1128 ± 2.3*
500
315 ± 2.35*
755 ± 1.68*
47 ± 2.67*
18 ± 1.98*
1596 ± 2.46*
1000
247 ± 2.38*
498 ± 1.78*
98 ± 1.6*
11 ± 1.61*
1489 ± 2.37*
2500
338 ± 2.35*
729 ± 1.57*
224 ± 2.32*
20 ± 3.33*
1438 ± 2.25*
Conditions: MSCs were incubated with 50 ng/mL–2500 ng/mL LPS for 3 h and then were lysed for mRNA extraction and qRT-PCR analysis. The presented data are statistically significant at the confidence level *P < 0.005, ().
