Role of Heparan Sulfate 2-O-Sulfotransferase in Prostate Cancer Cell Proliferation, Invasion, and Growth Factor Signaling
Figure 3
Inhibition by 2OST siRNA increases actin foci in C4-2B cells. (a) Fluorescence staining for F-actin (left panels) and nuclei (right panels) in C4-2B cells treated with 2OST siRNA (bottom row) or control siRNA (top row). Notice the clustering of F-actin into foci in the 2OST siRNA treated cells. (b) Quantitation of number of actin foci present per cell in C4-2B cells treated with scrambled siRNA (control, black bar) and 2OST siRNA (white bar). Mean number of foci per cell ± sem are and respectively (). Asterisk indicates . (c) Inhibition by 2OST siRNA increases E-cadherin staining that colocalizes with actin foci in C4-2B cells. Fluorescence immunohistochemistry for DAPI stained nuclei (left column); E-cadherin (second column), F-actin (third column), and merged images (fourth column) in C4-2B cells treated with 2OST siRNA (bottom row) or scrambled siRNA (top row). Notice accumulation of E-cadherin between cells treated with 2OST siRNA. (d) Inhibition by 2OST siRNA does not result in significant upregulation of E-cadherin protein. Western blot of E-cadherin in samples treated with scrambled siRNA or 2OST siRNA. Levels of E-cadherin were normalized to actin. Values represent two independent experiments.
