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1. F. Bray, J.-S. Ren, E. Masuyer, and J. Ferlay, “Global estimates of cancer prevalence for 27 sites in the adult population in 2008,” International Journal of Cancer, vol. 132, no. 5, pp. 1133–1145, 2013. View at Publisher · View at Google Scholar
2. J. E. Oesterling, “Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate,” Journal of Urology, vol. 145, no. 5, pp. 907–923, 1991. View at Scopus
3. W. J. Catalona, D. S. Smith, T. L. Ratliff et al., “Measurement of prostate-specific antigen in serum as a screening test for prostate cancer,” The New England Journal of Medicine, vol. 324, no. 17, pp. 1156–1161, 1991. View at Scopus
4. I. M. Thompson, D. K. Pauler, P. J. Goodman et al., “Prevalence of prostate cancer among men with a prostate-specific antigen level ≤ 4.0 ng per milliliter,” The New England Journal of Medicine, vol. 350, no. 22, pp. 2239–2346, 2004. View at Publisher · View at Google Scholar · View at Scopus
5. J. Hugosson, S. Carlsson, G. Aus et al., “Mortality results from the Göteborg randomised population-based prostate-cancer screening trial,” The Lancet Oncology, vol. 11, no. 8, pp. 725–732, 2010. View at Publisher · View at Google Scholar · View at Scopus
6. F. H. Schröder, J. Hugosson, M. J. Roobol et al., “Screening and prostate-cancer mortality in a randomized european study,” The New England Journal of Medicine, vol. 360, no. 13, pp. 1320–1328, 2009. View at Publisher · View at Google Scholar · View at Scopus
7. G. L. Andriole, E. D. Crawford, R. L. Grubb III et al., “Mortality results from a randomized prostate-cancer screening trial,” The New England Journal of Medicine, vol. 360, no. 13, pp. 1310–1319, 2009. View at Publisher · View at Google Scholar
8. M. Sánchez-Chapado, G. Olmedilla, M. Cabeza, E. Donat, and A. Ruiz, “Prevalence of prostate cancer and prostatic intraepithelial neoplasia in Caucasian Mediterranean males: an autopsy study,” Prostate, vol. 54, no. 3, pp. 238–247, 2003. View at Publisher · View at Google Scholar · View at Scopus
9. K. Stamatiou, A. Alevizos, E. Agapitos, and F. Sofras, “Incidence of impalpable carcinoma of the prostate and of non-malignant and precarcinomatous lesions in Greek male population: an autopsy study,” Prostate, vol. 66, no. 12, pp. 1319–1328, 2006. View at Publisher · View at Google Scholar · View at Scopus
10. P. C. Albertsen, J. A. Hanley, D. F. Gleason, and M. J. Barry, “Competing risk analysis of men aged 55 to 74 years at diagnosis managed conservatively for clinically localized prostate cancer,” Journal of the American Medical Association, vol. 280, no. 11, pp. 975–980, 1998. View at Publisher · View at Google Scholar · View at Scopus
11. P. Mufarrij, A. Sankin, G. Godoy, and H. Lepor, “Pathologic outcomes of candidates for active surveillance undergoing radical prostatectomy,” Urology, vol. 76, no. 3, pp. 689–692, 2010. View at Publisher · View at Google Scholar
12. G. Attard and J. S. De Bono, “Translating scientific advancement into clinical benefit for castration-resistant prostate cancer patients,” Clinical Cancer Research, vol. 17, no. 12, pp. 3867–3875, 2011. View at Publisher · View at Google Scholar · View at Scopus
13. K. A. Phillips, S. Van Bebber, and A. M. Issa, “Diagnostics and biomarker development: priming the pipeline,” Nature Reviews Drug Discovery, vol. 5, no. 6, pp. 463–469, 2006. View at Publisher · View at Google Scholar · View at Scopus
14. R. C. Lee, R. L. Feinbaum, and V. Ambros, “The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14,” Cell, vol. 75, no. 5, pp. 843–854, 1993. View at Publisher · View at Google Scholar · View at Scopus
15. D. P. Bartel, “MicroRNAs: target recognition and regulatory functions,” Cell, vol. 136, no. 2, pp. 215–233, 2009. View at Publisher · View at Google Scholar · View at Scopus
16. J. Winter, S. Jung, S. Keller, R. I. Gregory, and S. Diederichs, “Many roads to maturity: microRNA biogenesis pathways and their regulation,” Nature Cell Biology, vol. 11, no. 3, pp. 228–234, 2009. View at Publisher · View at Google Scholar · View at Scopus
17. Y. Lee, C. Ahn, J. Han et al., “The nuclear RNase III Drosha initiates microRNA processing,” Nature, vol. 425, no. 6956, pp. 415–419, 2003. View at Publisher · View at Google Scholar · View at Scopus
18. E. Lund, S. Güttinger, A. Calado, J. E. Dahlberg, and U. Kutay, “Nuclear export of microRNA precursors,” Science, vol. 303, no. 5654, pp. 95–98, 2004. View at Publisher · View at Google Scholar · View at Scopus
19. R. Yi, Y. Qin, I. G. Macara, and B. R. Cullen, “Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs,” Genes and Development, vol. 17, no. 24, pp. 3011–3016, 2003. View at Publisher · View at Google Scholar · View at Scopus
20. R. C. Friedman, K. K. H. Farh, C. B. Burge, and D. P. Bartel, “Most mammalian mRNAs are conserved targets of microRNAs,” Genome Research, vol. 19, no. 1, pp. 92–105, 2009. View at Publisher · View at Google Scholar · View at Scopus
21. B. P. Lewis, C. B. Burge, and D. P. Bartel, “Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets,” Cell, vol. 120, no. 1, pp. 15–20, 2005. View at Publisher · View at Google Scholar · View at Scopus
22. A. Kozomara and S. Griffiths-Jones, “MiRBase: integrating microRNA annotation and deep-sequencing data,” Nucleic Acids Research, vol. 39, no. 1, pp. D152–D157, 2011. View at Publisher · View at Google Scholar · View at Scopus
23. R. Zhang and B. Su, “Small but influential: the role of microRNAs on gene regulatory network and 3′UTR evolution,” Journal of Genetics and Genomics, vol. 36, no. 1, pp. 1–6, 2009. View at Publisher · View at Google Scholar · View at Scopus
24. G. A. Calin, C. D. Dumitru, M. Shimizu et al., “Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 24, pp. 15524–15529, 2002. View at Publisher · View at Google Scholar · View at Scopus
25. C. M. Croce, “Causes and consequences of microRNA dysregulation in cancer,” Nature Reviews Genetics, vol. 10, no. 10, pp. 704–714, 2009. View at Publisher · View at Google Scholar · View at Scopus
26. A. Schaefer, C. Stephan, J. Busch, G. M. Yousef, and K. Jung, “Diagnostic, prognostic and therapeutic implications of microRNAs in urologic tumors,” Nature Reviews Urology, vol. 7, no. 5, pp. 286–297, 2010. View at Publisher · View at Google Scholar · View at Scopus
27. R. Ma, T. Jiang, and X. Kang, “Circulating microRNAs in cancer: origin, function and application,” Journal of Experimental and Clinical Cancer Research, vol. 31, no. 1, article 38, 2012. View at Publisher · View at Google Scholar
28. Y. Karube, H. Tanaka, H. Osada et al., “Reduced expression of Dicer associated with poor prognosis in lung cancer patients,” Cancer Science, vol. 96, no. 2, pp. 111–115, 2005. View at Publisher · View at Google Scholar · View at Scopus
29. M. S. Kumar, R. E. Pester, C. Y. Chen et al., “Dicer1 functions as a haploinsufficient tumor suppressor,” Genes and Development, vol. 23, no. 23, pp. 2700–2704, 2009. View at Publisher · View at Google Scholar · View at Scopus
30. R. Koesters, V. Adams, D. Betts et al., “Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression,” Genomics, vol. 61, no. 2, pp. 210–218, 1999. View at Publisher · View at Google Scholar · View at Scopus
31. L. Li, C. Yu, H. Gao, and Y. Li, “Argonaute proteins: potential biomarkers for human colon cancer,” BMC Cancer, vol. 10, article no. 38, 2010. View at Publisher · View at Google Scholar · View at Scopus
32. D. Qiao, A. M. Zeeman, W. Deng, L. H. J. Looijenga, and H. Lin, “Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas,” Oncogene, vol. 21, no. 25, pp. 3988–3999, 2002. View at Publisher · View at Google Scholar · View at Scopus
33. J. Lu, G. Getz, E. A. Miska et al., “MicroRNA expression profiles classify human cancers,” Nature, vol. 435, no. 7043, pp. 834–838, 2005. View at Publisher · View at Google Scholar · View at Scopus
34. M. V. Iorio, M. Ferracin, C. G. Liu et al., “MicroRNA gene expression deregulation in human breast cancer,” Cancer Research, vol. 65, no. 16, pp. 7065–7070, 2005. View at Publisher · View at Google Scholar · View at Scopus
35. M. V. Iorio, R. Visone, G. Di Leva et al., “MicroRNA signatures in human ovarian cancer,” Cancer Research, vol. 67, no. 18, pp. 8699–8707, 2007. View at Publisher · View at Google Scholar · View at Scopus
36. P. S. Mitchell, R. K. Parkin, E. M. Kroh et al., “Circulating microRNAs as stable blood-based markers for cancer detection,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 30, pp. 10513–10518, 2008. View at Publisher · View at Google Scholar · View at Scopus
37. L. A. Selth, S. Townley, J. L. Gillis et al., “Discovery of circulating microRNAs associated with human prostate cancer using a mouse model of disease,” International Journal of Cancer, vol. 131, no. 3, pp. 652–661, 2012. View at Publisher · View at Google Scholar
38. S. S. C. Chim, T. K. F. Shing, E. C. W. Hung et al., “Detection and characterization of placental microRNAs in maternal plasma,” Clinical Chemistry, vol. 54, no. 3, pp. 482–490, 2008. View at Publisher · View at Google Scholar · View at Scopus
39. C. H. Lawrie, S. Gal, H. M. Dunlop et al., “Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma,” British Journal of Haematology, vol. 141, no. 5, pp. 672–675, 2008. View at Publisher · View at Google Scholar · View at Scopus
40. L. A. Selth, W. D. Tilley, and L. M. Butler, “Circulating microRNAs: macro-utility as markers of prostate cancer?” Endocrine-Related Cancer, vol. 19, no. 4, pp. R99–R113, 2012. View at Publisher · View at Google Scholar
41. X. Chen, Y. Ba, L. Ma et al., “Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases,” Cell Research, vol. 18, no. 10, pp. 997–1006, 2008. View at Publisher · View at Google Scholar · View at Scopus
42. J. D. Arroyo, J. R. Chevillet, E. M. Kroh et al., “Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma,” Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 12, pp. 5003–5008, 2011. View at Publisher · View at Google Scholar · View at Scopus
43. H. Valadi, K. Ekström, A. Bossios, M. Sjöstrand, J. J. Lee, and J. O. Lötvall, “Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells,” Nature Cell Biology, vol. 9, no. 6, pp. 654–659, 2007. View at Publisher · View at Google Scholar · View at Scopus
44. K. C. Vickers, B. T. Palmisano, B. M. Shoucri, R. D. Shamburek, and A. T. Remaley, “MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins,” Nature Cell Biology, vol. 13, no. 4, pp. 423–433, 2011. View at Publisher · View at Google Scholar · View at Scopus
45. J. Skog, T. Würdinger, S. van Rijn et al., “Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers,” Nature Cell Biology, vol. 10, no. 12, pp. 1470–1476, 2008. View at Publisher · View at Google Scholar · View at Scopus
46. M. A. Cortez, C. Bueso-Ramos, J. Ferdin, G. Lopez-Berestein, A. K. Sood, and G. A. Calin, “MicroRNAs in body fluids-the mix of hormones and biomarkers,” Nature Reviews Clinical Oncology, vol. 8, no. 8, pp. 467–477, 2011. View at Publisher · View at Google Scholar · View at Scopus
47. Y. Zhang, D. Liu, X. Chen et al., “Secreted monocytic miR-150 enhances targeted endothelial cell migration,” Molecular Cell, vol. 39, no. 1, pp. 133–144, 2010. View at Publisher · View at Google Scholar · View at Scopus
48. J. A. Weber, D. H. Baxter, S. Zhang et al., “The microRNA spectrum in 12 body fluids,” Clinical Chemistry, vol. 56, no. 11, pp. 1733–1741, 2010. View at Publisher · View at Google Scholar · View at Scopus
49. M. Hanke, K. Hoefig, H. Merz et al., “A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer,” Urologic Oncology, vol. 28, no. 6, pp. 655–661, 2010. View at Publisher · View at Google Scholar · View at Scopus
50. N. J. Park, H. Zhou, D. Elashoff et al., “Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection,” Clinical Cancer Research, vol. 15, no. 17, pp. 5473–5477, 2009. View at Publisher · View at Google Scholar
51. M. J. Lodes, M. Caraballo, D. Suciu, S. Munro, A. Kumar, and B. Anderson, “Detection of cancer with serum miRNAs on an oligonucleotide microarray,” PLoS ONE, vol. 4, no. 7, Article ID e6229, 2009. View at Publisher · View at Google Scholar
52. F. Moltzahn, A. B. Olshen, L. Baehner et al., “Microfluidic-based multiplex qRT-PCR identifies diagnostic and prognostic microRNA signatures in the sera of prostate cancer patients,” Cancer Research, vol. 71, no. 2, pp. 550–560, 2011. View at Publisher · View at Google Scholar · View at Scopus
53. J. C. Brase, M. Johannes, T. Schlomm et al., “Circulating miRNAs are correlated with tumor progression in prostate cancer,” International Journal of Cancer, vol. 128, no. 3, pp. 608–616, 2011. View at Publisher · View at Google Scholar
54. J. C. Gonzales, L. M. Fink, O. B. Goodman Jr., J. T. Symanowski, N. J. Vogelzang, and D. C. Ward, “Comparison of circulating MicroRNA 141 to circulating tumor cells, lactate dehydrogenase, and prostate-specific antigen for determining treatment response in patients with metastatic prostate cancer,” Clinical Genitourinary Cancer, vol. 9, no. 1, pp. 39–45, 2011. View at Publisher · View at Google Scholar
55. F. Y. Agaoglu, M. Kovancilar, Y. Dizdar et al., “Investigation of miR-21, miR-141, and miR-221 in blood circulation of patients with prostate cancer,” Tumour Biology, vol. 32, no. 3, pp. 583–588, 2011. View at Publisher · View at Google Scholar · View at Scopus
56. R. Mahn, L. C. Heukamp, S. Rogenhofer, A. Von Ruecker, S. C. Müller, and J. Ellinger, “Circulating microRNAs (miRNA) in serum of patients with prostate cancer,” Urology, vol. 77, no. 5, pp. 1265–e9, 2011. View at Publisher · View at Google Scholar · View at Scopus
57. R. J. Bryant, T. Pawlowski, J. W. F. Catto et al., “Changes in circulating microRNA levels associated with prostate cancer,” British Journal of Cancer, vol. 106, no. 4, pp. 768–774, 2012. View at Publisher · View at Google Scholar
58. H. C. N. Nguyen, W. Xie, M. Yang et al., “Expression differences of circulating microRNAs in metastatic castration resistant prostate cancer and low-risk, localized prostate cancer,” Prostate, vol. 73, no. 4, pp. 346–354, 2013. View at Publisher · View at Google Scholar
59. Z.-H. Chen, G.-L. Zhang, H.-R. Li et al., “A panel of five circulating microRNAs as potential biomarkers for prostate cancer,” Prostate, vol. 72, no. 13, pp. 1443–1452, 2012. View at Publisher · View at Google Scholar
60. C. Zheng, S. Yinghao, and J. Li, “MiR-221 expression affects invasion potential of human prostate carcinoma cell lines by targeting DVL2,” Medical Oncology, vol. 29, no. 2, pp. 815–822, 2012. View at Publisher · View at Google Scholar
61. J. Shen, G. W. Hruby, J. M. McKiernan et al., “Dysregulation of circulating microRNAs and prediction of aggressive prostate cancer,” Prostate, vol. 72, no. 13, pp. 1469–1477, 2012. View at Publisher · View at Google Scholar
62. I. Sanders, S. Holdenrieder, G. Walgenbach-Brünagel et al., “Evaluation of reference genes for the analysis of serum miRNA in patients with prostate cancer, bladder cancer and renal cell carcinoma,” International Journal of Urology, vol. 19, no. 11, pp. 1017–1025, 2012. View at Publisher · View at Google Scholar
63. J. C. Brase, D. Wuttig, R. Kuner, and H. Sültmann, “Serum microRNAs as non-invasive biomarkers for cancer,” Molecular Cancer, vol. 9, article no. 306, 2010. View at Publisher · View at Google Scholar · View at Scopus
64. H. L. Zhang, L. F. Yang, Y. Zhu et al., “Serum miRNA-21: elevated levels in patients with metastatic hormone-refractory prostate cancer and potential predictive factor for the efficacy of docetaxel-based chemotherapy,” Prostate, vol. 71, no. 3, pp. 326–331, 2011. View at Publisher · View at Google Scholar · View at Scopus
65. D. Hessels, J. M. T. Klein Gunnewiek, I. Van Oort et al., “DD${\text{3}}^{\text{PCA3}}$-based molecular urine analysis for the diagnosis of prostate cancer,” European Urology, vol. 44, no. 1, pp. 8–16, 2003. View at Publisher · View at Google Scholar
66. J. Szczyrba, E. Löprich, S. Wach et al., “The microRNA profile of prostate carcinoma obtained by deep sequencing,” Molecular Cancer Research, vol. 8, no. 4, pp. 529–538, 2010. View at Publisher · View at Google Scholar · View at Scopus
67. H. Cheng, L. Zhang, D. E. Cogdell et al., “Circulating plasma MiR-141 is a novel biomarker for metastatic colon cancer and predicts poor prognosis,” PLoS One, vol. 6, no. 3, Article ID e17745, 2011. View at Publisher · View at Google Scholar · View at Scopus
68. L. M. Li, Z. B. Hu, Z. X. Zhou et al., “Serum microRNA profiles serve as novel biomarkers for HBV infection and diagnosis of HBV-positive hepatocarcinoma,” Cancer Research, vol. 70, no. 23, pp. 9798–9807, 2010. View at Publisher · View at Google Scholar · View at Scopus
69. R. Duttagupta, R. Jiang, J. Gollub, R. C. Getts, and K. W. Jones, “Impact of cellular miRNAs on circulating miRNA biomarker signatures,” PLoS One, vol. 6, no. 6, Article ID e20769, 2011. View at Publisher · View at Google Scholar · View at Scopus
70. J. S. McDonald, D. Milosevic, H. V. Reddi, S. K. Grebe, and A. Algeciras-Schimnich, “Analysis of circulating microRNA: preanalytical and analytical challenges,” Clinical Chemistry, vol. 57, no. 6, pp. 833–840, 2011. View at Publisher · View at Google Scholar · View at Scopus
71. M. B. Kirschner, S. C. Kao, J. J. Edelman et al., “Haemolysis during sample preparation alters microRNA content of plasma,” PLoS One, vol. 6, no. 9, Article ID e24145, 2011. View at Publisher · View at Google Scholar
72. L. J. Sokoll, W. Ellis, P. Lange et al., “A multicenter evaluation of the PCA3 molecular urine test: pre-analytical effects, analytical performance, and diagnostic accuracy,” Clinica Chimica Acta, vol. 389, no. 1-2, pp. 1–6, 2008. View at Publisher · View at Google Scholar · View at Scopus
73. E. M. Kroh, R. K. Parkin, P. S. Mitchell, and M. Tewari, “Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR),” Methods, vol. 50, no. 4, pp. 298–301, 2010. View at Publisher · View at Google Scholar · View at Scopus
74. L. W. Lee, S. Zhang, A. Etheridge et al., “Complexity of the microRNA repertoire revealed by next-generation sequencing,” RNA, vol. 16, no. 11, pp. 2170–2180, 2010. View at Publisher · View at Google Scholar · View at Scopus
75. B. M. Ryan, A. I. Robles, and C. C. Harris, “Genetic variation in microRNA networks: the implications for cancer research,” Nature Reviews Cancer, vol. 10, no. 6, pp. 389–402, 2010. View at Publisher · View at Google Scholar · View at Scopus
76. Z. Huang, D. Huang, S. Ni, Z. Peng, W. Sheng, and X. Du, “Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer,” International Journal of Cancer, vol. 127, no. 1, pp. 118–126, 2010. View at Publisher · View at Google Scholar · View at Scopus
77. B. M. Bolstad, R. A. Irizarry, M. Åstrand, and T. P. Speed, “A comparison of normalization methods for high density oligonucleotide array data based on variance and bias,” Bioinformatics, vol. 19, no. 2, pp. 185–193, 2003. View at Publisher · View at Google Scholar · View at Scopus
78. A. Deo, J. Carlsson, and A. Lindlöf, “How to choose a normalization strategy for miRNA quantitative real-time (QPCR) arrays,” Journal of Bioinformatics and Computational Biology, vol. 9, no. 6, pp. 795–812, 2011. View at Publisher · View at Google Scholar
79. Y. J. Hua, K. Tu, Z. Y. Tang, Y. X. Li, and H. S. Xiao, “Comparison of normalization methods with microRNA microarray,” Genomics, vol. 92, no. 2, pp. 122–128, 2008. View at Publisher · View at Google Scholar · View at Scopus
80. S. Pradervand, J. Weber, J. Thomas et al., “Impact of normalization on miRNA microarray expression profiling,” RNA, vol. 15, no. 3, pp. 493–501, 2009. View at Publisher · View at Google Scholar · View at Scopus
81. M. S. Pepe, R. Etzioni, Z. Feng et al., “Phases of biomarker development for early detection of cancer,” Journal of the National Cancer Institute, vol. 93, no. 14, pp. 1054–1061, 2001. View at Scopus
82. R. Simon, M. D. Radmacher, K. Dobbin, and L. M. McShane, “Pitfalls in the use of DNA microarray data for diagnostic and prognostic classification,” Journal of the National Cancer Institute, vol. 95, no. 1, pp. 14–18, 2003. View at Scopus