763569.fig.005a
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763569.fig.005b
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Figure 5: AR-target genes promote cell invasion in an androgen-dependent manner in AA cancer cells. (a) Androgen stimulation (10 nM DHT for 48 hr) increases invasion in the AA PCa cell lines MDA PCa 2b and E006AA. (b) siRNA-mediated knockdown of AR-target genes RHOA, ITGB5, or PIK3CD diminished androgen-induced (10 nM DHT for 48 hr) cell invasion in both MDA PCa 2b and E006AA cell lines. Knockdown efficiency (determined for each experiment) was typically ~80% as determined by qRT-PCR (data not shown). Data are represented as the mean ± SEM of 4–7 independent cell invasion assays. *Significant difference between cells treated with vehicle (<0.01% ethanol final concentration) versus 10 nM DHT using an ANOVA with Holm post hoc test ( ). **Significant difference between vehicle-treated cells incubated with siRNA against RHOA, ITGB5, or PIK3CB versus vehicle-treated cells incubated with nonsense siRNA (siNS) using an ANOVA with Holm post hoc test ( ). ***Significant difference between DHT-stimulated cells incubated with siRNA against RHOA, ITGB5, or PIK3CB versus DHT-stimulated cells incubated with nonsense siRNA (siNS) using an ANOVA with Holm post hoc test ( ).