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Prostate Cancer
Volume 2013 (2013), Article ID 763569, 15 pages
http://dx.doi.org/10.1155/2013/763569
Research Article

Androgen Receptor-Target Genes in African American Prostate Cancer Disparities

1Department of Pharmacology and Physiology, The George Washington University Medical Center, Washington, DC 20037, USA
2Medical Faculty Associates, The George Washington University Medical Center, Washington, DC 20037, USA
3Division of Urology, Mount Sinai Medical Center, Columbia University, Miami Beach, FL 33140, USA
4The GW Cancer Institute, The George Washington University Medical Center, Washington, DC 20037, USA
5Duke Cancer Institute, Duke University Medical Center, Durham, NC 27710, USA

Received 21 September 2012; Revised 14 December 2012; Accepted 18 December 2012

Academic Editor: Craig Robson

Copyright © 2013 Bi-Dar Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Material includes Supplemental Tables S1 to S6 and Supplemental Figures S1 to S8. The Supplemental Tables are described as follows:

Supplemental Table S1: Primer sequences for qRT-PCRs, Supplemental Table S2: Primer sequences for ChIP-PCR validation of AR target genes, Supplemental Table S3: Differentially expressed genes between AA cancer versus AA matched normal, CA cancer versus CA matched normal, AA cancer versus CA cancer, and genes in common or unique in the pairwise comparisons, Supplemental Table S4: Ingenuity canonical pathways significantly over-represented in AA cancer but not in CA cancer, Supplemental Table S5: Putative AR target genes in AA PCa, Supplemental Table S6: IPA canonical signaling pathways with over-represented AR target genes in the comparison of AA PCa versus CA PCa

Supplemental Figures are described as follows:

Supplemental Figure S1: Principal component analysis (PCA) of prostate tissue specimens based on mRNA expression, Supplemental Figure S2: Over-representation of differentially expressed genes in the AR signaling pathway of AA PCa specimens, Supplemental Figure S3: Venn diagram depicting differentially expressed genes derived from pairwise comparisons of AA cancer versus AA matched normal, CA cancer versus CA matched normal, and AA cancer versus CA cancer, Supplemental Figure S4: Canonical signaling pathways with a significant over-representation of differentially expressed AR-target genes, Supplemental Figure S5: Western blot analysis of AR protein levels in CA PCa cell line VCaP and AA PCa cell lines E006AA and MDA PCa 2b, Supplemental Figure S6: DHT-stimulated gene expression after 24-hr and 48-hr serum starvation, Supplemental Figure S7: Effect of DHT treatment on AR mRNA expression in AA PCa cell lines, Supplemental Figure S8: Knockdown efficiencies of siRNAs targeting RHOA, ITGB5 and PIK3CB in PCa cells.

  1. Supplementary Material