Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice

Figure 1

Western blot and probe localization. Human A431, MCF7, U87 GM, PC3 M-LN4, and 22Rv1 cell lysates were prepared for western blot analysis (a) to confirm PSMA reactivity of anti-PSMA antibody candidate. PSMA 800CW distinguished between 22Rv1 (PSMA positive) and PC3 M-LN4 (PSMA negative) prostate tumor cell lysates, with no signal detected for cell lysates of nonprostate origin (A431, MCF-7, and U-87 MG). Microscopy images captured cell binding for PSMA 800CW ((b) and (c)) and YC-27 800CW ((d) and (e)) to 22Rv1 (PSMA positive, (b) and (d)) and PC3 M-LN4 (PSMA negative, (c) and (d)). Images were obtained by fluorescence microscopy using an Olympus IX81 Inverted Microscope.