Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice
Figure 2
Immunofluorescent cell-based comparison of binding and inhibition. (a) 22Rv1 and PC3 M-LN4 cells were incubated with YC-27 800CW (1 nM to 0.5 μM). Apparent = 8 nM. for PC3 M-LN4 could not be determined. (b) LNCaP cells were incubated with YC-27 800CW (0.5 nM to 0.5 μM, apparent = 36 nM). Competition of YC-27 800CW binding to LNCaP cells by unlabeled YC-27 ((c), IC50 = 1.7 μM) or inhibition by 2-PMPA preincubation ((d), IC50 = 0.1 μM).
