Role of p73 Dinucleotide Polymorphism in Prostate Cancer and p73 Protein Isoform Balance

Table 3

p73 DNP genotypes and p73 isoform expression ratios for cancer cell lines.

Cell line	p73 DNP genotype	TAp73/ΔNp73^∗
HCT116	GC/GC	0.68
HeLa	GC/GC	0.95
H460	GC/GC	0.74
HEK293	GC/GC	0.72
MCF7	GC/GC	0.97
H1299	GC/GC	0.69
JEKO-1	GC/GC	0.84
BEAS-2B	GC/GC	0.86
Jurkat	GC/AT	1.40
HepG2	GC/AT	1.39
CaCO-2	AT/AT	N/A

Cell lines were used for genotyping for p73 DNP status and western analyses for p73 protein N-terminal isoforms and beta-actin. *TAp73/ΔNp73 protein isoform levels were determined from the western data in Figures 2(a) and 2(b) by quantifying protein bands using ImageJ64. Relative TAp73/ΔNp73 protein isoform ratios were corrected for using actin levels as loading controls and were calculated using the formula: TAp73/ΔNp73 = ((TAp73/actin)/(ΔNp73/actin)).