424285.fig.002a
(a) MA-induced Time dependent changes in GSH levels
424285.fig.002b
(b) Effect of NAC on MA-induced depletion in GSH levels
Figure 2: Effect of NAC on MA-induced reduction in total GSH levels. (a) Determination of cellular GSH. N27 dopaminergic cells were treated with 2 mM MA for 3, 6, 12, 18, and 24 hours. MA-induced reduction in GSH levels was measured by the monochlorobimane fluorometric method. The data represent mean ± SEM of six individual measurements. Asterisks (*** ) indicate significant differences between MA-treated cells and untreated control cells. Values are expressed as percentage of GSH compared with untreated control. Treatment of N27 dopaminergic cells with 5 μM MG132 is considered as positive control. (b) Determination of cellular GSH levels in N27 dopaminergic cells preincubated (1 hour) with 5 mM NAC prior to MA treatment for 18 hours. The data represent mean ± SEM of four individual measurements. Asterisks (* ) indicate significant differences between MA-treated cells and NAC alone or NAC with MA-treated cells. Values are expressed as percentage of GSH compared with untreated cells.