- About this Journal ·
- Abstracting and Indexing ·
- Advance Access ·
- Aims and Scope ·
- Annual Issues ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Volume 2012 (2012), Article ID 920953, 13 pages
Dopamine Oxidation and Autophagy
1Molecular & Clinical Pharmacology, ICBM, Faculty of Medicine, University of Chile, Independencia 1027, Santiago 8380453, Chile
2Department of Basic Sciences, Santo Tomas University, Viña del Mar 2561780, Chile
Received 5 June 2012; Accepted 9 July 2012
Academic Editor: José Manuel Fuentes Rodríguez
Copyright © 2012 Patricia Muñoz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The molecular mechanisms involved in the neurodegenerative process of Parkinson's disease remain unclear. Currently, there is a general agreement that mitochondrial dysfunction, α-synuclein aggregation, oxidative stress, neuroinflammation, and impaired protein degradation are involved in the neurodegeneration of dopaminergic neurons containing neuromelanin in Parkinson's disease. Aminochrome has been proposed to play an essential role in the degeneration of dopaminergic neurons containing neuromelanin by inducing mitochondrial dysfunction, oxidative stress, the formation of neurotoxic α-synuclein protofibrils, and impaired protein degradation. Here, we discuss the relationship between the oxidation of dopamine to aminochrome, the precursor of neuromelanin, autophagy dysfunction in dopaminergic neurons containing neuromelanin, and the role of dopamine oxidation to aminochrome in autophagy dysfunction in dopaminergic neurons. Aminochrome induces the following: (i) the formation of α-synuclein protofibrils that inactivate chaperone-mediated autophagy; (ii) the formation of adducts with α- and β-tubulin, which induce the aggregation of the microtubules required for the fusion of autophagy vacuoles and lysosomes.
1. Dopamine Synthesis and Degradation
Dopamine is a neurotransmitter that plays an essential role in the control of movements and loss of dopaminergic neurons containing neuromelanin in the nigrostriatal system. In addition, dopamine is involved in the development of motor symptoms experienced in patients diagnosed with Parkinson’s disease (PD). Dopamine is synthesized in a sequential reaction in which the cytosolic enzymes tyrosine hydroxylase (TH) and aromatic amino acid decarboxylase (AADC) catalyze the hydroxylation of the amino acid tyrosine to L-dihydroxyphenylanaline (L-dopa) and decarboxylation of L-dopa to dopamine, respectively. The protons of the hydroxyl group in dopamine dissociate when dopamine is localized in the cytosol at physiological pH. However, these protons are tightly bound to the hydroxyl group once dopamine is inside monoaminergic synaptic vesicles, which have a relatively low pH. The membrane of monoaminergic synaptic vesicles contains a vesicular monoaminergic transporter-2 (VMAT-2) that catalyzes the uptake of dopamine into these vesicles. These monoaminergic synaptic vesicles contain an ATPase that hydrolyzes ATP to ADP and Pi, and one proton (H+) is translocated into the vesicle, generating a proton gradient. VMAT-2 uses this proton gradient to take up one molecule of dopamine with the concomitant release of two protons [1, 2]. The increase of protons inside monoaminergic synaptic vesicles induces a decrease in the pH of the vesicle, which is estimated to be 2 to 2.4 pH units lower than that of the cytosol . TH and AADC have been shown to associate with monoaminergic synaptic vesicles containing VMAT-2  by forming a complex. Tyrosine is then converted to L-dopa and immediately decarboxylated to dopamine, preventing the presence of free dopamine in the cytosol (Figure 1).
Dopamine in the cytosol spontaneously oxidizes to aminochrome without metal-ion catalysis . Thus, VMAT-2 plays an important role in preventing the oxidation of dopamine in dopaminergic neurons. Other enzymes that prevent dopamine oxidation to aminochrome are monoamino oxidase (MAO) and catechol ortho-methyl transferase (COMT). MAO degrades excess dopamine in the cytosol by catalyzing the oxidative deamination of the amino group of dopamine to 3,4-dihydroxyphenylacetaldehyde with the concomitant formation of an ammonium molecule and hydrogen peroxide. Aldehyde dehydrogenase can then convert 3,4-dihydroxyphenylacetaldehyde to 3,4-dihydroxyphenylacetic acid (DOPAC), which can be converted to homovanillic acid catalyzed by COMT. Dopamine can also be methylated by COMT, generating 3-methoxytyramine, which can be converted to 3-methoxy-4-hydroxyphenylacetaldehyde, hydrogen peroxide, and NH3 by the enzyme MAO. Finally, the enzyme aldehyde dehydrogenase catalyzes the conversion of 3-methoxy-4-hydroxyphenylacetaldehyde to homovanillic acid (Figure 2). MAO enzymes are localized in the outer membranes of mitochondria in neurons, glial cells, and other cell types [6, 7]. MAO-A is mostly localized in catecholaminergic neurons, whereas MAO-B is found in serotonergic and histaminergic neurons as well as astrocytes . COMT has two isoforms, one soluble (S-COMT) and one membrane-bound (MB-COMT) isoform. Both isoforms are found in microglial, astroglial, and some neuronal cells, such as pyramidal neurons, cerebellar Purkinje and granular cells, and striatal spiny neurons . However, dopamine still oxidizes to aminochrome, even in the presence of VMAT-2, MAO-A, and S-COMT, which prevent the existence of free dopamine in the cytosol. Aminochrome, the precursor to neuromelanin, is a dark pigment found in dopaminergic neurons localized in the substantia nigra.
2. Dopamine Oxidation
Free cytosolic dopamine has protons that dissociate from their corresponding hydroxyl groups, promoting the oxidation of dopamine to dopamine o-quinone. This oxidation can proceed via a one-electron oxidation of dopamine to form a dopamine o-semiquinone radical (reaction 1), which is subsequently oxidized to dopamine o-quinone (reaction 2) by reducing two molecules of oxygen to superoxide radicals. The dopamine o-semiquinone radical does not strongly react with oxygen, leading to the formation of leukoaminochrome o-semiquinone radical during a one-electron reduction of aminochrome . Subsequently, two dopamine o-semiquinone radicals can disproportionate, generating one molecule of dopamine o-quinone and one molecule of dopamine (reaction 3). A two-electron oxidation of dopamine to dopamine o-quinone is catalyzed by the enzyme tyrosinase. Notably, the presence of dopamine o-semiquinone is not detected by electron spin resonance . Dopamine o-quinone is not stable in the cytosol at physiological pH and its amino chain cyclizes (reaction 5), generating aminochrome (Figure 3). Dopamine o-quinone has been reported to form adducts with parkin, mitochondrial complex I and III, and dopamine transporters [12–14]. However, the molecule actually that forms these adducts is aminochrome because dopamine o-quinone is only stable below pH 2.0 . Aminochrome is formed by the oxidation of dopamine by tyrosinase, which can be further purified by chromatography, and it is stable for approximately 3 hours .
The oxidation of dopamine can also be catalyzed by enzymes with peroxidase activity, such as prostaglandin H synthase, cytochrome P450 forms, dopamine β-mono-oxygenase, and xanthine oxidase [16–20]. Lactoperoxidase catalyzes the one-electron oxidation of dopamine to a dopamine o-semiquinone radical, which was confirmed by electron spin resonance . However, dopamine can also be oxidized by metals, such as manganese, copper, iron, or sodium metaperiodate [11, 21–24]. At physiological pH, dopamine o-quinone is a transient product because it is unstable above pH 2, resulting in the further oxidation of dopamine . Dopamine o-quinone rearranges by cyclizing its amino chain to form aminochrome (reactions 5 and 6). These proteins are inactivated by aminochrome because dopamine o-quinone cyclizes immediately at physiological pH .
3. Aminochrome Metabolism
3.1. Formation of Neuromelanin
Aminochrome is the precursor to neuromelanin because neuromelanin formation is dependent on the rearrangement of aminochrome to 5,6-dihydroxyindole, which is then oxidized to 5,6-indolequinone followed by further polymerization to form neuromelanin  (Figure 4). Postmortem studies using healthy subjects have shown that neuromelanin formation is a normal process in substantia nigra. Furthermore, this pigment is located in intact dopaminergic neurons because it is formed during the overtime and accumulates with age . Neuromelanin acts as a chelator for metals [27, 28], indicating that this molecule plays a neuroprotective role. Neuromelanin accumulates in double membrane vacuoles, preventing neurotoxic effects of free neuromelanin in cells exposed to this pigment [29, 30].
3.2. Formation of Aminochrome and Protein Adducts
Aminochrome forms adducts with proteins such as α-synuclein , stabilizing and inducing the formation of neurotoxic protofibrils . In familiar PD, the formation of neurotoxic α-synuclein protofibers is dependent on a specific point mutation . However, in sporadic PD, the formation of neurotoxic protofibrils appears to be dependent on the ability of aminochrome to form α-synuclein protofibrils. Aminochrome is also able to form adducts with mitochondrial complexes I and III, as well as isocitrate dehydrogenase , suggesting that this molecule induces mitochondrial dysfunction and a subsequent collapse in energy. Aminochrome also forms adducts with the protein DJ-1 , which has been suggested to be involved in the regulation of mitochondrial dynamics. Overexpression of the DJ-1 mutant associated with PD induces a significant increase in fragmented mitochondria, mitochondrial dysfunction, and increased neuronal vulnerability to oxidative stress .
Aminochrome has been reported to disrupt the architecture of the cytoskeleton in cell cultures  by forming aggregates with actin and α- and β-tubulin. Other studies also report the formation of aminochrome adducts with actin and β-tubulin . In addition, aminochrome has been shown to form adducts with the ubiquitin carboxy-terminal hydrolase isoenzyme L1 (UCH-L1) , which was determined to be associated with familiar PD by a gene mutation (Figure 5).
3.3. One-Electron Reduction of Aminochrome
Aminochrome can undergo a one-electron reduction by flavoenzymes that utilize NADH or NADPH as an electron donator to generate a leukoaminochrome-o-semiquinone radical. This radical is extremely reactive with oxygen and autoxidizes to aminochrome under aerobic conditions. Molecular oxygen is then reduced to superoxide radicals, generating a redox cycle between the leukoaminochrome o-semiquinone radical and aminochrome [10, 36]. The redox cycling between aminochrome and leukoaminochrome o-semiquinone radical plays an important role in aminochrome neurotoxicity because it induces an energy collapse when flavoenzymes utilize NADH, which is required for ATP synthesis in the mitochondria [37, 38]. The use of NADPH in redox cycling also affects the cell because NADPH is required to catalyze the reduction of oxidized glutathione by glutathione reductase, which is an important antioxidant. The neurotoxic effects of this redox cycling are enhanced by the dismutation of superoxide radicals to hydrogen peroxide, the precursor of hydroxyl radicals. The one-electron reduction of aminochrome has been reported to be neurotoxic to catecholaminergic cells [15, 21, 22, 38–45] (Figure 6).
3.4. Two-Electron Reduction of Aminochrome
Aminochrome can undergo a two-electron reduction by DT-diaphorase (EC.220.127.116.11), a flavoenzyme that uses both NADH and NADPH as electron donors, and the product of this reaction is the hydroquinone leukoaminochrome . Leukoaminochrome can autoxidize in the presence of superoxide radicals. However, the presence of superoxide dismutase in the cytosol prevents leukoaminochrome autoxidation from occurring . We have proposed a protective role for DT-diaphorase against aminochrome neurotoxicity, which is supported by cell culture studies based on the inhibition of DT-diaphorase with dicoumarol and the induced aminochrome neurotoxicity that results from reduced DT-diaphorase expression by siRNA [38, 41, 42, 46]. DT-diaphorase also prevents the formation of α-synuclein protofibrils [47, 48] and disruption of the cytoskeleton, which is generally the consequence of forming adducts with actin and α- and β-tubulin . DT diaphorase immunoreactivity has been observed in dopaminergic neurons and Bergmann glia, astrocytes, and tanycytes  (Figure 7).
3.5. Aminochrome Conjugation with Glutathione
Aminochrome can be conjugated with glutathione by glutathione S-transferase M2-2 (GST M2-2) to 4-S-glutathionyl-5,6-dihydroxyindoline. 4-S-Glutathionyl-5,6-dihydroxyindoline is stable in the presence of biological oxidizing agents, such as oxygen, superoxide radicals, and hydrogen peroxide [50, 51]. The stability of 4-S-glutathionyl-5,6-dihydroxyindoline in the presence of biological oxidizing agents suggest that is a final elimination product. Interestingly, the precursor of aminochrome, dopamine o-quinone, is also conjugated by GST M2-2 to 5-glutathionyl-dopamine, preventing the formation of aminochrome . All glutathione conjugates undergo degradation of the tripeptide γ-L-Glu-L-Cys-Gly, named the glutathione to cysteil conjugate. Thus, 5-glutathionyl-dopamine is converted to 5-cysteinyl dopamine. Notably, 5-S-cysteinyl-dopamine has been detected in the cerebrospinal fluid of PD patients, dopamine-rich regions of the brain such as the caudate nucleus, putamen, globus pallidus and substantia nigra, and in neuromelanin [53–55]. GST M2-2 also catalyzes the conjugation of dopa o-quinone to 5-glutathionyl dopa leads to the degradation of the tripeptide glutathione, generating 5-cysteinyl dopa . Melanoma cells produce and release 5-cysteinyl-dopamine, which is then excreted through the urine . Thus, the conjugation of glutathione must be a protective reaction against aminochrome neurotoxicity (Figure 8).
4. PD and Autophagy
Autophagy is an important intracellular bulk degradation and recycling process in which cytoplasmic proteins and organelles accumulate in autophagy vacuoles that are transported into lysosomes [58–60]. Autophagy plays an important role in the elimination of damaged organelles, such as the mitochondria. Autophagy dysfunction has been speculated to play an important role in the pathogenesis of PD . Wild-type α-synuclein is degraded by chaperone-mediated autophagy (CMA) and macroautophagy because the inhibition of CMA and macroautophagy lead to accumulation of wild type α-synuclein . The expression of the α-synuclein mutant A53T induces CMA dysfunction, which is mediated by the expression of α-synuclein protofibrils . Interestingly, the pathogenic A53T and A30P α-synuclein mutants inhibit their own degradation and that of other substrates . Overexpression of the α-synuclein mutant A53T in transgenic animals demonstrates that A53T localizes to mitochondrial membranes as a monomer, inhibiting complex I and increasing mitochondrial autophagy . α-synuclein impairs autophagy via Rab1a inhibition, and Rab1a overexpression rescues the autophagy defect caused by α-synuclein . A mutation in ubiquitin C-terminal hydrolase L1 (UCH-L1), which is associated with familial PD, was reported to inhibit CMA autophagy by interacting with the lysosomal receptor of CMA LAMP-2A . Parkin has also been shown to promote autophagy of damaged mitochondria by relocalizing into dysfunctional mitochondria with low membrane potentials in mammalian cells . The loss of DJ-1induces a reduction in the mitochondrial membrane potential and an increase in the fragmentation and accumulation of autophagy markers. These effects appear to be mediated by oxidative stress because supplementing DJ-1-deficient cells with glutathione has been shown to reverse these effects on mitochondria and autophagy . Transfection of the common G2019S LRRK2 mutation into SH-SY5Y cells was reported to increase autophagy in both neuritic and somatic compartments . Autophagy activation was observed to restore the mitochondrial membrane potential impaired by rotenone in SH-SY5Y cell lines overexpressing α-synuclein  and attenuate rotenone-induced toxicity in SH-SY5Y cell lines .
5. Aminochrome and Autophagy
In Parkinson’s disease, autophagy dysfunction plays an important role in the neurodegeneration of dopaminergic neurons containing neuromelanin. Proteins associated with familial PD have been reported to play a role in autophagy dysfunction, such as α-synuclein, UCH-L1, and DJ-1 [63, 67, 69]. Mutated α-synuclein (A53T) generates protofibrils that inhibit CMA autophagy. As a result, dopamine-modified α-synuclein is poorly degraded by CMA and also inhibits the degradation of other substrates using this pathway . Aminochrome was reported to form adducts with α-synuclein by binding to the 125YEMPS129 motif of α-synuclein and inducing and stabilizing the formation of protofibrils . These observations suggest that aminochrome is involved in the alpha synuclein-dependent inhibition of CMA autophagy because aminochrome induces the formation of α-synuclein protofibrils, such as the A53T mutant (Figure 10). Mutated UCH-L1 also inhibits CMA autophagy by interacting with the lysosomal receptor for CMA LAMP-2A . Aminochrome has been shown to also form adducts with UCH-L1 . Little is known about the effects of the aminochrome-induced modification of UCH-L1. However, we speculate that the aminochrome-induced modification of UCH-L1 also impairs CMA autophagy. In addition, aminochrome forms adducts with the protein DJ-1, and the loss of DJ-1 indirectly alters autophagy by interfering with the regulation of oxidative stress .
Microtubules are an important component of the cytoskeleton, which are composed by subunits of α- and β-tubulin and normally exist as dimers. Microtubules also play a role in the formation of autophagosomes and fusion of autophagosomes with lysosomes [74, 75]. Aminochrome forms adducts with β-tubulin  and the aminochrome one-electron reduction product when DT-diaphorase is inhibited by dicoumarol. This inhibition leads to the disruption of the cytoskeleton by disrupting the α- and β-tubulin network and its aggregation around the cell membrane (; Figure 9). Thus, we speculate that aminochrome prevents the fusion of autophagosomes with lysosomes by inducing the aggregation of microtubules. We hypothesize that the number of autophagosomes will increase in the cytosol when aminochrome inhibits the fusion between autophagosomes and lysosomes by preventing the formation of normal microtubules because of the formation of aminochrome adducts with α- or β-tubulin (Figure 10). Incubation of RCSN-3 cells with 20 μM aminochrome in either the presence or absence of 100 μM dicoumarol induced a significant increase in the number of autophagosomes in the cytosol (6- and 9-fold, resp.; Figure 9). These results support aminochrome playing a role in autophagy dysfunction. Aminochrome forms adducts with α- or β-tubulin, preventing the fusion of autophagosomes and lysosomes that lead to an increase in the number of autophagosomes in the cytosol . Furthermore, these data support the observation that aminochrome induces a significant increase of GFP-LC3 positive staining in cells treated with aminochrome . Aminochrome has also been reported to inactivate parkin, an ubiquitin ligase of the proteasomal system, by forming adducts with parkin  as well as inhibit the proteasome . All of these results support the involvement of aminochrome in the dysfunction of protein degradation.
This work was supported by FONDECYT Grant 1100165 (Chile). The authors thank Dr. Pablo Caviedes, who provided the cell line RCSN-3 in the experiment done in Figure 9. For any request of this cell line, please contact him directly by e-mail: firstname.lastname@example.org.
- F. A. Chaudhry, R. H. Edwards, and F. Fonnum, “Vesicular neurotransmitter transporters as targets for endogenous and exogenous toxic substances,” Annual Review of Pharmacology and Toxicology, vol. 48, pp. 277–301, 2008.
- J. Knoth, M. Zallakian, and D. Njus, “Stoichiometry of H+-linked dopamine transport in chromaffin granule ghosts,” Biochemistry, vol. 20, no. 23, pp. 6625–6629, 1981.
- T. S. Guillot and G. W. Miller, “Protective actions of the vesicular monoamine transporter 2 (VMAT2) in monoaminergic neurons,” Molecular Neurobiology, vol. 39, no. 2, pp. 149–170, 2009.
- E. A. Cartier, L. A. Parra, T. B. Baust et al., “A biochemical and functional protein complex involving dopamine synthesis and transport into synaptic vesicles,” Journal of Biological Chemistry, vol. 285, no. 3, pp. 1957–1966, 2010.
- W. Linert, E. Herlinger, R. F. Jameson, E. Kienzl, K. Jellinger, and M. B. H. Youdim, “Dopamine, 6-hydroxydopamine, iron, and dioxygen—their mutual interactions and possible implication in the development of Parkinson's disease,” Biochimica et Biophysica Acta, vol. 1316, no. 3, pp. 160–168, 1996.
- W. Weyler, Y. P. Hsu, and X. O. Breakefield, “Biochemistry and genetics of monoamine oxidase,” Pharmacology and Therapeutics, vol. 47, no. 3, pp. 391–417, 1990.
- J. C. Shih, J. Grimsby, and K. Chen, “Molecular biology of monoamine oxidase A and B: their role in the degradation of serotonin,” in Handbook of Experimental Pharmacology, H. G. Baumgarten and M. Gothert, Eds., vol. 129 of Serotoninergic Neurons and 5-HT receptors in the CNS, pp. 655–670, Springer, Berlin, Germany, 1997.
- K. N. Westlund, R. M. Denney, R. M. Rose, and C. W. Abell, “Localization of distinct monoamine oxidase A and monoamine oxidase B cell populations in human brainstem,” Neuroscience, vol. 25, no. 2, pp. 439–456, 1988.
- T. T. Myöhänen, N. Schendzielorz, and P. T. Männistö, “Distribution of catechol-O-methyltransferase (COMT) proteins and enzymatic activities in wild-type and soluble COMT deficient mice,” Journal of Neurochemistry, vol. 113, no. 6, pp. 1632–1643, 2010.
- J. Segura-Aguilar, D. Metodiewa, and C. J. Welch, “Metabolic activation of dopamine o-quinones to o-semiquinones by NADPH cytochrome P450 reductase may play an important role in oxidative stress and apoptotic effects,” Biochimica et Biophysica Acta, vol. 1381, no. 1, pp. 1–6, 1998.
- J. Segura-Aguilar and C. Lind, “On the mechanism of the Mn3+-induced neurotoxicity of dopamine: prevention of quinone-derived oxygen toxicity by DT diaphorase and superoxide dismutase,” Chemico-Biological Interactions, vol. 72, no. 3, pp. 309–324, 1989.
- Y. Xu, A. H. Stokes, R. Roskoski Jr., and K. E. Vrana, “Dopamine, in the presence of tyrosinase, covalently modifies and inactivates tyrosine hydroxylase,” Journal of Neuroscience Research, vol. 54, pp. 691–697, 1998.
- R. E. Whitehead, J. V. Ferrer, J. A. Javitch, and J. B. Justice, “Reaction of oxidized dopamine with endogenous cysteine residues in the human dopamine transporter,” Journal of Neurochemistry, vol. 76, no. 4, pp. 1242–1251, 2001.
- M. J. LaVoie, B. L. Ostaszewski, A. Weihofen, M. G. Schlossmacher, and D. J. Selkoe, “Dopamine covalently modifies and functionally inactivates parkin,” Nature Medicine, vol. 11, no. 11, pp. 1214–1221, 2005.
- I. Paris, C. Perez-Pastene, S. Cardenas et al., “Aminochrome induces disruption of actin, alpha-, and beta-tubulin cytoskeleton networks in substantia-nigra-derived cell line,” Neurotoxicity Research, vol. 18, no. 1, pp. 82–92, 2010.
- T. G. Hastings, “Enzymatic oxidation of dopamine: the role of prostaglandin H synthase,” Journal of Neurochemistry, vol. 64, no. 2, pp. 919–924, 1995.
- J. Segura-Aguilar, “Peroxidase activity of liver microsomal vitamin D 25-hydroxylase and cytochrome P450 1A2 catalyzes 25-hydroxylation of vitamin D3 and oxidation of dopamine to aminochrome,” Biochemical and Molecular Medicine, vol. 58, no. 1, pp. 122–129, 1996.
- C. Foppoli, R. Coccia, C. Cini, and M. A. Rosei, “Catecholamines oxidation by xanthine oxidase,” Biochimica et Biophysica Acta, vol. 1334, no. 2-3, pp. 200–206, 1997.
- L. Galzigna, A. De Iuliis, and L. Zanatta, “Enzymatic dopamine peroxidation in substantia nigra of human brain,” Clinica Chimica Acta, vol. 300, no. 1-2, pp. 131–138, 2000.
- C. M. Thompson, J. H. Capdevila, and H. W. Strobel, “Recombinant cytochrome P450 2D18 metabolism of dopamine and arachidonic acid,” Journal of Pharmacology and Experimental Therapeutics, vol. 294, no. 3, pp. 1120–1130, 2000.
- I. Paris, A. Dagnino-Subiabre, K. Marcelain et al., “Copper neurotoxicity is dependent on dopamine-mediated copper uptake and one-electron reduction of aminochrome in a rat substantia nigra neuronal cell line,” Journal of Neurochemistry, vol. 77, no. 2, pp. 519–529, 2001.
- I. Paris, P. Martinez-Alvarado, S. Cárdenas et al., “Dopamine-dependent iron toxicity in cells derived from rat hypothalamus,” Chemical Research in Toxicology, vol. 18, no. 3, pp. 415–419, 2005.
- W. H. Harrison, W. W. Whisler, and B. J. Hill, “Catecholamine oxidation and ionization properties indicated from the H+ release, tritium exchange, and spectral changes which occur during ferricyanide oxidation,” Biochemistry, vol. 7, no. 9, pp. 3089–3094, 1968.
- D. G. Graham, S. M. Tiffany, W. R. Bell Jr., and W. F. Gutknecht, “Autoxidation versus covalent binding of quinones as the mechanism of toxicity of dopamine, 6-hydroxydopamine, and related compounds toward C1300 neuroblastoma cells in vitro,” Molecular Pharmacology, vol. 14, no. 4, pp. 644–653, 1978.
- A. Napolitano, P. Manini, and M. d'Ischia, “Oxidation chemistry of catecholamines and neuronal degeneration: an update,” Current Medicinal Chemistry, vol. 18, no. 12, pp. 1832–1845, 2011.
- L. Zecca, R. Fariello, P. Riederer, D. Sulzer, A. Gatti, and D. Tampellini, “The absolute concentration of nigral neuromelanin, assayed by a new sensitive method, increases throughout the life and is dramatically decreased in Parkinson's disease,” FEBS Letters, vol. 510, no. 3, pp. 216–220, 2002.
- M. Gerlach, K. L. Double, D. Ben-Shachar, L. Zecca, M. B. H. Youdim, and P. Riederer, “Neuromelanin and its interaction with iron as a potential risk factor for dopaminergic neurodegeneration underlying Parkinson's disease,” Neurotoxicity Research, vol. 5, no. 1-2, pp. 35–43, 2003.
- M. Fasano, B. Bergamasco, and L. Lopiano, “Is neuromelanin changed in Parkinson's disease? Investigations by magnetic spectroscopies,” Journal of Neural Transmission, vol. 113, no. 6, pp. 769–774, 2006.
- M. Naoi, W. Maruyama, H. Yi et al., “Neuromelanin selectively induces apoptosis in dopaminergic SH-SY5Y cells by deglutathionylation in mitochondria: involvement of the protein and melanin component,” Journal of Neurochemistry, vol. 105, no. 6, pp. 2489–2500, 2008.
- W. Zhang, K. Phillips, A. R. Wielgus et al., “Neuromelanin activates microglia and induces degeneration of dopaminergic neurons: implications for progression of parkinson's disease,” Neurotoxicity Research, vol. 19, no. 1, pp. 63–72, 2011.
- E. H. Norris, B. I. Giasson, R. Hodara et al., “Reversible inhibition of α-synuclein fibrillization by dopaminochrome-mediated conformational alterations,” Journal of Biological Chemistry, vol. 280, no. 22, pp. 21212–21219, 2005.
- K. A. Conway, J. C. Rochet, R. M. Bieganski, and P. T. Lansbury Jr., “Kinetic stabilization of the α-synuclein protofibril by a dopamine-α-synuclein adduct,” Science, vol. 294, no. 5545, pp. 1346–1349, 2001.
- M. H. Polymeropoulos, C. Lavedan, E. Leroy et al., “Mutation in the α-synuclein gene identified in families with Parkinson's disease,” Science, vol. 276, no. 5321, pp. 2045–2047, 1997.
- V. S. Van Laar, A. J. Mishizen, M. Cascio, and T. G. Hastings, “Proteomic identification of dopamine-conjugated proteins from isolated rat brain mitochondria and SH-SY5Y cells,” Neurobiology of Disease, vol. 34, no. 3, pp. 487–500, 2009.
- X. Wang, T. G. Petrie, Y. Liu, J. Liu, H. Fujioka, and X. Zhu, “Parkinson's disease-associated DJ-1 mutations impair mitochondrial dynamics and cause mitochondrial dysfunction,” Journal of Neurochemistry, vol. 121, no. 5, pp. 830–839, 2012.
- S. Baez, Y. Linderson, and J. Segura-Aguilar, “Superoxide dismutase and catalase enhance autoxidation during one-electron reduction of aminochrome by NADPH-cytochrome P-450 reductase,” Biochemical and Molecular Medicine, vol. 54, no. 1, pp. 12–18, 1995.
- P. Aguirre, P. Urrutia, V. Tapia et al., “The dopamine metabolite aminochrome inhibits mitochondrial complex I and modifies the expression of iron transporters DMT1 and FPN1,” Biometals, vol. 25, no. 4, pp. 795–803, 1995.
- P. Muñoz, I. Paris, L. H. Sanders, J. T. Greenamyre, and J. Segura-Aguilar, “Overexpression of VMAT-2 and DT-diaphorase protects substantia nigra-derived cells against aminochrome neurotoxicity,” Biochimica et Biophysica Acta, vol. 1822, no. 7, pp. 1125–1136, 2012.
- I. Paris, P. Martinez-Alvarado, C. Perez-Pastene et al., “Monoamine transporter inhibitors and norepinephrine reduce dopamine-dependent iron toxicity in cells derived from the substantia nigra,” Journal of Neurochemistry, vol. 92, no. 5, pp. 1021–1032, 2005.
- I. Paris, C. Perez-Pastene, E. Couve, P. Caviedes, S. LeDoux, and J. Segura-Aguilar, “Copper·dopamine complex induces mitochondrial autophagy preceding caspase-independent apoptotic cell death,” Journal of Biological Chemistry, vol. 284, no. 20, pp. 13306–13315, 2009.
- I. Paris, P. Muñoz, S. Huenchuguala et al., “Autophagy protects against aminochrome-induced cell death in substantia nigra-derived cell line,” Toxicological Sciences, vol. 121, no. 2, pp. 376–388, 2011.
- C. Arriagada, I. Paris, M. J. Sanchez de las Matas et al., “On the neurotoxicity mechanism of leukoaminochrome o-semiquinone radical derived from dopamine oxidation: mitochondria damage, necrosis, and hydroxyl radical formation,” Neurobiology of Disease, vol. 16, no. 2, pp. 468–477, 2004.
- P. Fuentes, I. Paris, M. Nassif, P. Caviedes, and J. Segura-Aguilar, “Inhibition of VMAT-2 and DT-diaphorase induce cell death in a substantia nigra-derived cell line—an experimental cell model for dopamine toxicity studies,” Chemical Research in Toxicology, vol. 20, no. 5, pp. 776–783, 2007.
- G. Díaz-Véliz, I. Paris, S. Mora, R. Raisman-Vozari, and J. Segura-Aguilar, “Copper neurotoxicity in rat substantia Nigra and striatum is dependent on DT-diaphorase inhibition,” Chemical Research in Toxicology, vol. 21, no. 6, pp. 1180–1185, 2008.
- P. Muñoz, S. Huenchuguala, I. Paris et al., “Protective effects of nicotine against aminochrome-induced toxicity in substantia nigra derived cells: implications for Parkinson's disease,” Neurotoxicity Research, vol. 22, no. 2, pp. 177–180, 2012.
- J. Lozano, P. Muñoz, B. F. Nore, S. Ledoux, and J. Segura-Aguilar, “Stable expression of short interfering RNA for DT-diaphorase induces neurotoxicity,” Chemical Research in Toxicology, vol. 23, no. 9, pp. 1492–1496, 2010.
- J. Segura-Aguilar, S. Cardenas, A. Riveros et al., “DT-diaphorase prevents the formation of alpha-synuclein adducts with aminochrome,” Society for Neuroscience, vol. 824, article 17, 2006.
- S. P. Cardenas, C. Perez-Pastene, E. Couve, and J. Segura-Aguilar, “The DT-diaphorase prevents the aggregation of a-synuclein induced by aminochrome,” Neurotoxicity Research, vol. 13, article 136, 2008.
- M. Schultzberg, J. Segura-Aguilar, and C. Lind, “Distribution of DT diaphorase in the rat brain: biochemical and immunohistochemical studies,” Neuroscience, vol. 27, no. 3, pp. 763–776, 1988.
- J. Segura-Aguilar, S. Baez, M. Widersten, C. J. Welch, and B. Mannervik, “Human class Mu glutathione transferases, in particular isoenzyme M2-2, catalyze detoxication of the dopamine metabolite aminochrome,” Journal of Biological Chemistry, vol. 272, no. 9, pp. 5727–5731, 1997.
- S. Baez, J. Segura-Aguilar, M. Widersten, A. S. Johansson, and B. Mannervik, “Glutathione transferases catalyse the detoxication of oxidized metabolites (o-quinones) of catecholamines and may serve as an antioxidant system preventing degenerative cellular processes,” Biochemical Journal, vol. 324, no. 1, pp. 25–28, 1997.
- X. M. Shen, B. Xia, M. Z. Wrona, and G. Dryhurst, “Synthesis, redox properties, in vivo formation, and neurobehavioral effects of N-acetylcysteinyl conjugates of dopamine: possible metabolites of relevance to Parkinson's disease,” Chemical Research in Toxicology, vol. 9, no. 7, pp. 1117–1126, 1996.
- F. C. Cheng, J. S. Kuo, L. G. Chia, et al., “Elevated 5-S-cysteinyldopamine/homovanillic acid ratio and reduced homovanillic acid in cerebrospinal fluid: possible markers for and potential insights into the pathoetiology of Parkinson's disease,” Journal of Neural Transmission, vol. 103, no. 4, pp. 433–446, 1996.
- E. Rosengren, E. Linder-Eliasson, and A. Carlsson, “Detection of 5-S-cysteinyldopamine in human brain,” Journal of Neural Transmission, vol. 63, no. 3-4, pp. 247–253, 1985.
- R. Carstam, C. Brinck, A. Hindemith-Augustsson, H. Rorsman, and E. Rosengren, “The neuromelanin of the human substantia nigra,” Biochimica et Biophysica Acta, vol. 1097, no. 2, pp. 152–160, 1991.
- A. Dagnino-Subiabre, B. K. Cassels, S. Baez, A. S. Johansson, B. Mannervik, and J. Segura-Aguilar, “Glutathione transferase M2-2 catalyzes conjugation of dopamine and dopa o-quinones,” Biochemical and Biophysical Research Communications, vol. 274, no. 1, pp. 32–36, 2000.
- S. Ito, T. Kato, K. Maruta, et al., “Determination of DOPA, dopamine, and 5-S-cysteinyl-DOPA in plasma, urine, and tissue samples by high-performance liquid chromatography with electrochemical detection,” Journal of Chromatography, vol. 311, no. 1, pp. 154–159, 1984.
- Y. Matsui, S. Kyoi, H. Takagi et al., “Molecular mechanisms and physiological significance of autophagy during myocardial ischemia and reperfusion,” Autophagy, vol. 4, no. 4, pp. 409–415, 2008.
- G. Tettamanti, E. Saló, C. González-Estévez, D. A. Felix, A. Grimaldi, and M. de Eguileor, “Autophagy in invertebrates: insights into development, regeneration and body remodeling,” Current Pharmaceutical Design, vol. 14, no. 2, pp. 116–125, 2008.
- A. Kuma and N. Mizushima, “Physiological role of autophagy as an intracellular recycling system: with an emphasis on nutrient metabolism,” Seminars in Cell and Developmental Biology, vol. 21, no. 7, pp. 683–690, 2010.
- M. A. Lynch-Day, K. Mao, K. Wang, M. Zhao, and D. J. Klionsky, “The role of autophagy in Parkinson's disease,” Cold Spring Harbor Perspectives in Medicine, vol. 2, no. 4, Article ID a009357, pp. 1–13, 2012.
- T. Vogiatzi, M. Xilouri, K. Vekrellis, and L. Stefanis, “Wild type α-synuclein is degraded by chaperone-mediated autophagy and macroautophagy in neuronal cells,” Journal of Biological Chemistry, vol. 283, no. 35, pp. 23542–23556, 2008.
- M. Xilouri, T. Vogiatzi, K. Vekrellis, D. Park, and L. Stefanis, “Abberant α-synuclein confers toxicity to neurons in part through inhibition of chaperone-mediated autophagy,” PLoS ONE, vol. 4, no. 5, Article ID e5515, 2009.
- A. M. Cuervo, L. Stafanis, R. Fredenburg, P. T. Lansbury Jr., and D. Sulzer, “Impaired degradation of mutant α-synuclein by chaperone-mediated autophagy,” Science, vol. 305, no. 5688, pp. 1292–1295, 2004.
- S. J. Chinta, J. K. Mallajosyula, A. Rane, and J. K. Andersen, “Mitochondrial alpha-synuclein accumulation impairs complex I function in dopaminergic neurons and results in increased mitophagy in vivo,” Neuroscience Letters, vol. 486, no. 3, pp. 235–239, 2010.
- A. R. Winslow, C. W. Chen, S. Corrochano et al., “α-Synuclein impairs macroautophagy: implications for Parkinson's disease,” Journal of Cell Biology, vol. 190, no. 6, pp. 1023–1037, 2010.
- T. Kabuta, A. Furuta, S. Aoki, K. Furuta, and K. Wada, “Aberrant interaction between Parkinson disease-associated mutant UCH-L1 and the lysosomal receptor for chaperone-mediated autophagy,” Journal of Biological Chemistry, vol. 283, no. 35, pp. 23731–23738, 2008.
- D. Narendra, A. Tanaka, D. F. Suen, and R. J. Youle, “Parkin is recruited selectively to impaired mitochondria and promotes their autophagy,” Journal of Cell Biology, vol. 183, no. 5, pp. 795–803, 2008.
- M. K. McCoy and M. R. Cookson, “DJ-1 regulation of mitochondrial function and autophagy through oxidative stress,” Autophagy, vol. 7, no. 5, pp. 531–532, 2011.
- E. D. Plowey, S. J. Cherra III, Y. J. Liu, and C. T. Chu, “Role of autophagy in G2019S-LRRK2-associated neurite shortening in differentiated SH-SY5Y cells,” Journal of Neurochemistry, vol. 105, no. 3, pp. 1048–1056, 2008.
- S. Dadakhujaev, H. S. Noh, E. J. Jung et al., “Autophagy protects the rotenone-induced cell death in α-synuclein overexpressing SH-SY5Y cells,” Neuroscience Letters, vol. 472, no. 1, pp. 47–52, 2010.
- N. Xiong, M. Jia, C. Chen et al., “Potential autophagy enhancers attenuate rotenone-induced toxicity in SH-SY5Y,” Neuroscience, vol. 199, pp. 292–302, 2011.
- M. Martinez-Vicente, Z. Talloczy, S. Kaushik et al., “Dopamine-modified α-synuclein blocks chaperone-mediated autophagy,” Journal of Clinical Investigation, vol. 118, no. 2, pp. 777–778, 2008.
- R. Köchl, X. W. Hu, E. Y. W. Chan, and S. A. Tooze, “Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes,” Traffic, vol. 7, no. 2, pp. 129–145, 2006.
- L. Jahreiss, F. M. Menzies, and D. C. Rubinsztein, “The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes,” Traffic, vol. 9, no. 4, pp. 574–587, 2008.
- K. S. Zafar, D. Siegel, and D. Ross, “A potential role for cyclized quinones derived from dopamine, DOPA, and 3,4-dihydroxyphenylacetic acid in proteasomal inhibition,” Molecular Pharmacology, vol. 70, no. 3, pp. 1079–1086, 2006.