Magnolol Protects against MPTP/MPP+-Induced Toxicity via Inhibition of Oxidative Stress in In Vivo and In Vitro Models of Parkinson’s Disease
Figure 2
Effect of pre-MPTP treatment with magnolol on DAT, TH and GFAP protein levels in the striatum of C57BL/6 mice. Mice were administered daily with magnolol (30 mg/kg) for 5 days, and received 4 injections of 20 mg/kg MPTP at 2 h intervals at the day after the last magnolol treatment. The evaluations were performed at 3 days after MPTP treatment. Representative immunoblots of DAT, TH and GFAP protein in the striatum are shown (a). Actin protein was used as housekeeping protein. Immunoblots of DAT (b), TH (c), and GFAP (d) in the striatum were quantified in each group. Densitometric analysis of protein bands were performed using software (Image J 1.36, NIH). The data were expressed as % of control. Results are means ± SD (-7) from two independent experiments. Statistical comparisons were carried out by one-way ANOVA followed by Scheffe test. *** versus control, # versus MPTP + vehicle.
