Comparative Proteomic Analysis of Carbonylated Proteins from the Striatum and Cortex of Pesticide-Treated Mice

Figure 5

KEGG pathways enriched for carbonylated proteins by pesticide treatment and brain region. The carbonylated proteins identified in two mouse brain regions, striatum (ST) and cortex (CTX), were functionally annotated using DAVID Tools. KEGG pathways were examined for overrepresentation in carbonylated protein lists obtained for each of the four treatment groups, maneb (MB), paraquat (PQ), paraquat and maneb combined (PQMB), and controls, and in each brain region separately. KEGG pathways that were nominally significant () in at least one of the 8 protein lists and not in all 8 protein lists are included in the graphic. The colors of the heatmap range from white () to bright red based on the negative log base 10 transformation of the value. A value of 1 was used when the KEGG pathway was represented by less than two proteins in the list. KEGG pathways (rows) are ordered based on hierarchical clustering where the distance measure was calculated as one minus the Spearman correlation coefficient when values were converted to a binary indicator of significance ( versus ).