Hepcidin Plays a Key Role in 6-OHDA Induced Iron Overload and Apoptotic Cell Death in a Cell Culture Model of Parkinson’s Disease

Figure 1

Effect of hepcidin knockdown on hepcidin mRNA levels measured by quantitative real-time RT-PCR ((a), -8), ferroportin 1 protein levels (normalized to β-actin) measured by Western blot (b), and intracellular iron measured by a calcein quenching method ((c), ) in N27 cells. Representative calcein fluorescence images with and without incubation of 1 mM ferrous sulfate for 30 min are shown (d). Values are mean ± SEM. Differences between two groups were based on Student’s -test; ; . Control siRNA: scrambled small interfering RNA; hepcidin siRNA: hepcidin small interfering RNA; Fpn1: ferroportin 1.

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