Comparative mRNA Expression of eEF1A Isoforms and a PI3K/Akt/mTOR Pathway in a Cellular Model of Parkinson’s Disease

Figure 2

Effect of MPP⁺ on cell viability of nondifferentiated SH-SY5Y cells (a) and effect of MPP⁺ on cell viability of differentiated SH-SY5Y cells (b). Cells were exposed to 125, 250, 500, 1000, and 2000 μM of MPP⁺ for 24 hours and cell viability was measured with an MTT assay. For neuronal differentiation, SH-SY5Y cells were treated with 10 μM retinoic acid for three days before exposure to MPP⁺. The control groups for nondifferentiated and differentiated SH-SY5Y cells were treated with the same medium without MPP⁺. MTT assays were repeated three times in triplicate measurement. Data are shown as mean ± SEM. indicates and indicates versus 0 μm of MPP⁺.

(a)

(b)