Altered Responses to Cold Environment in Urocortin 1 and Corticotropin-Releasing Factor Deficient Mice

Table 4

Expression levels of transcripts measured by real-time polymerase chain reaction (RT-PCR) from total RNA prepared from brown adipose tissues (BAT), skeletal muscles, kidney, subcutaneous fat (SQ fat), liver, and heart. The data are expressed as means ± SEM. 𝑃 values of UCN1 KO versus WT were calculated using Student’s 𝑡-test. BAT: brown adipose tissue; UCP1: uncoupling protein 1; THR-α: thyroid hormone receptor-α subunit; THR-β: thyroid hormone receptor-β subunit; AdpR1: adiponectin receptor 1; AdpR2: adiponectin receptor 2; CRFR2: corticotropin-releasing hormone receptor 2.

Gene	Tissue	Sex	Genotype	RQ	(𝑛)	𝑃 values
UCP1	BAT	Females	WT	0.74 ± 0.38	6	0.493
			UCN1KO	0.44 ± 0.23	8
		Males	WT	3.10 ± 1.53	9	0.802
			UCN1KO	2.61 ± 1.11	9
THR-α	Skeletal muscle	Females	WT	2.22 ± 0.93	6	0.731
			UCN1KO	1.74 ± 0.96	7
		Males	WT	2.40 ± 1.30	8	0.959
			UCN1KO	2.49 ± 0.94	6
THR-β	Kidney	Females	WT	0.42 ± 0.15	6	0.864
			UCN1KO	0.39 ± 0.11	8
		Males	WT	2.98 ± 0.86	9	0.884
			UCN1KO	3.19 ± 1.16	7
Adiponectin	SQ fat	Females	WT	1.25 ± 0.23	6	0.547
			UCN1KO	1.48 ± 0.28	6
		Males	WT	1.00 ± 0.30	8	0.273
			UCN1KO	1.78 ± 0.64	7
AdpR1	Skeletal muscle	Females	WT	2.00 ± 0.29	6	0.014
			UCN1KO	1.09 ± 0.16	8
		Males	WT	0.56 ± 0.13	9	0.905
			UCN1KO	0.60 ± 0.28	7
AdpR2	Liver	Females	WT	0.58 ± 0.10	6	0.176
			UCN1KO	0.37 ± 0.10	8
		Males	WT	0.67 ± 0.12	9	0.175
			UCN1KO	1.46 ± 0.54	9
CRFR2	Heart	Females	WT	0.28 ± 0.15	6	0.368
			UCN1KO	0.96 ± 0.65	7
		Males	WT	4.04 ± 1.21	8	0.319
			UCN1KO	6.14 ± 1.70	6