Gradual Increase of High Mobility Group Protein B1 in the Lungs after the Onset of Acute Exacerbation of Idiopathic Pulmonary Fibrosis
Figure 5
Distribution of alveolar capillary endothelial cells positive for thrombomodulin in stable IPF, acute exacerbation of IPF and controls. (a) and (b) The lung tissues which were surgically biopsied from a patient with stable IPF were double immunostained for HMGB1 (in brown) and CD34 (in red) (a). The consecutive sections were stained for HMGB1 (in brown) and thrombomodulin (in red) (b) (bars = 100 μm). (c) and (d) The lung tissues which were obtained by autopsy from a patient who died after acute exacerbation were double immunostained for HMGB1 (in brown) and CD34 (in red) (c) or thrombomodulin (in red) (d) in the consecutive sections (bars = 100 μm). (e) The percentages of the distribution areas of the capillary endothelial cells immunoreactive for thrombomodulin per those immunoreactive for CD34 were evaluated by a digital image analyzer and compared among stable IPF (), acute exacerbation (AEx IPF, ) of IPF, and controls ().
