Research Article

The Effects of Gas Humidification with High-Flow Nasal Cannula on Cultured Human Airway Epithelial Cells

Figure 3

Cell viability for Calu-3 monolayers exposed to one of three levels of relative humidity: <20% (dry), 69% (noninterventional comparator), and >90% (HFNC). CellTiter Blue cell viability assay was performed to estimate the number of viable cells and determine cell viability by the intensity of fluorescence. Cell viability decreased over time ( ๐‘ƒ < 0 . 0 0 1 ) for HFNC and dry groups. Data are mean ยฑ SEM. *Group effect ( ๐‘ƒ < 0 . 0 5 ). ยงTime effect ( ๐‘ƒ < 0 . 0 5 ). ๐‘› = 6 /condition.
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