The Effects of Gas Humidification with High-Flow Nasal Cannula on Cultured Human Airway Epithelial Cells
Figure 6
Cytomorphological examination of Calu-3 cell monolayers exposed to one of three levels of relative humidity: <20% (dry), 69% (noninterventional comparator), and >90% (HFNC). Representative cytomorphological examination of Calu-3 cells for both the noninterventional comparator group and the HFNC group demonstrated normal morphology. At 4 and 8 hours, the dry group showed abnormal cellular appearances, swollen nuclei, intracellular and nuclear vacuoles, diffused cytoplasm, and cellular debris. All cytospins were examined by light microscopy at 40x magnification.
