|
| Gene | Primer sequences (5′-3′) | Length (bp) |
(°C) |
|
| -Actin | Sense: AACCCTAAGGCCAACCGTGAAAAG | 343 | 57 |
| Antisense: GCTCGAAGTCTAGGGCAACATA |
|
| PDE1 | Sense: TGGAAGCTGCACTACAGGTG | 375 | 59 |
| Antisense: CTTCAGGTCCACAGCTGACA |
|
| PDE2 | Sense: GAGGACATCGAGATCTTTGC | 442 | 57 |
| Antisense: TCTTTGTAGATCAGCTCCGC |
|
| PDE3 | Sense: TCTTTGCCACTCCTACGACT | 265 | 57 |
| Antisense: CTGTGCCTGATAAACACTGC |
|
| PDE4A | Sense: TCAACACCAATTCGGAGCTGG | 216 | 61 |
| Antisense: GTCTTCAGGTCAGCCAGGAGG |
|
| PDE4B | Sense: AGGATTCTGAAGGACCGG | 154 | 56 |
| Antisense: AGATTATGTGTCGATCAG |
|
| PDE4C | Sense: ACTGAGTCTGCGCAGGATGG | 539 | 62 |
| Antisense: CACTCCTCTTCCTCTGCTCTCCTC |
|
| PDE4D | Sense: GGCTTCATAGACTACATTG | 418 | 56 |
| Antisense: TTACACTGTTACGTGTCAGG |
|
| PDE5 | Sense: CTGTCTGATCTGGAAACAGC | 250 | 57 |
| Antisense: GCAATCAGCAATGCAAGCGT |
|
| PDE7 | Sense: TCGTATGCTAGGAGATGTCCG | 334 | 55 |
| Antisense: GCTTACTAGACTATTTCCATTTG |
|
| PDE8 | Sense: GGAGAACCAACTCCTTCCTGTG | 592 | 58 |
| Antisense: AGGCATCCCATGCATCAAAC |
|
| PDE9 | Sense: ATGGACCGAGACAAAGTGAC | 275 | 57 |
| Antisense: AGGCGAACGGTCTTCATTGT |
|
| PDE10 | Sense: CTGAGGGGGATGAGATGAAG | 322 | 58 |
| Antisense: TCAGTTGCTAGGCAGACATCA |
|
| PDE11 | Sense: TTCAGCTCGGACAGTCCTAAA | 374 | 58 |
| Antisense: TCCACTAGCAAAGGAGACGAA |
|
