PPAR/-Independent Effects of PPAR/ Ligands on Cysteinyl Leukotriene Production in Mast Cells
Figure 3
Measurement
of mRNA levels of PPAR (upper panel) and PPAR (lower panel) with real-time semiquantitative PCR. Total RNA
(1 g) extracted from
white adipose tissue (), liver (), BMMC (), and RBL-2H3 mast cells () was supplemented with 50 pg of chloramphenicol
acetyltransferase RNA and then reverse-transcribed. The indicated amounts of
cDNA were applied to real-time PCR. PCR performed without cDNA was used as a
negative control () of the reaction. Data are presented as the number of PCR
cycles to cross the threshold.
Messenger RNA levels in these tissues
were extrapolated from the PCR cycle of the liver for PPARα or
white adipose tissue for PPAR and then corrected by the
chloramphenicol acetyltransferase cDNA content in each sample and presented in
the manuscripts [13, 14].
), liver (), BMMC (), and RBL-2H3 mast cells () was supplemented with 50 pg of chloramphenicol
acetyltransferase RNA and then reverse-transcribed. The indicated amounts of
cDNA were applied to real-time PCR. PCR performed without cDNA was used as a
negative control () of the reaction. Data are presented as the number of PCR
cycles to cross the threshold.
Messenger RNA levels in these tissues
were extrapolated from the PCR cycle of the liver for PPARα or
white adipose tissue for PPAR and then corrected by the
chloramphenicol acetyltransferase cDNA content in each sample and presented in
the manuscripts [
