Microglia and Astrocyte Activation by Toll-Like Receptor Ligands: Modulation by PPAR- Agonists
Figure 10
15d- is a global inhibitor of astrocytic
IL-12 p40 release following TLR ligand exposure. Primary astrocytes were
plated at cells/well in 96-well plates and incubated
overnight. The following day, cells were pretreated for 1 hour with the
indicated concentrations of 15d- prior to the addition of TLR
ligands. Cell-conditioned supernatants were collected at 24 hours following TLR
ligand exposure, whereupon IL-12 p40 levels were determined by ELISA (a) and (b).
Significant differences between astrocytes treated with TLR ligands alone
versus TLR ligands + 15d-PGJ2 are noted with asterisks . Results
presented are representative of two independent experiments.
