Microglia and Astrocyte Activation by Toll-Like Receptor Ligands: Modulation by PPAR- Agonists
Figure 6
15d-PGJ2 demonstrates differential effects on
CXCL2 production by microglia, which are TLR ligand-dependent. Primary
microglia were plated at cells/well in 96-well plates and
incubated overnight. The following day, cells were pretreated for 1 hour with
the indicated concentrations of 15d- prior to the addition of
TLR ligands. Cell-conditioned supernatants were collected at 24 hours following
TLR ligand exposure, whereupon CXCL2 levels were determined by ELISA.
Significant differences between microglia treated with TLR ligands alone versus
TLR ligands + 15d-PGJ2 are noted with asterisks . Results
presented are representative of two independent experiments.
