]>PPAR Action in Human Placental Development and Pregnancy and Its Complications : Figure 3
127048.fig.003
Figure 3: Immunoblot of JEG-3 cells treated with pooled sera (10%) from nonpregnant (NP), pregnant (P), and preeclamptic (PE) women. Cell lysates were analyzed using a specific mouse anti-human PPARγ monoclonal antibody. Equal amounts of protein (50 μg) were loaded into each lane. Factors in pregnant serum up-regulate JEG-3 PPARγ expression. A decrease in PPARγ protein was observed in cells exposed to PE sera (PE) compared to sera from normal pregnant women (P).