Regulation of Peroxisome Proliferator-Activated Receptors by E6-Associated Protein
Figure 5
E6-AP is induced by
clofibrate in a PPAR-dependent
manner. Wildtype and PPAR
null mice were treated with control vehicle or clofibrate for 2 weeks. Groups
of five mice were used for each treatment. (a) Total RNA was isolated from liver and mRNA levels were
measured using real-time PCR. (b)
Protein isolated from liver was analyzed for E6AP expression by western
blot. The graph (lower panel) depicts
mean band intensity. Asterisks indicate a significant increase in
clofibrate induction when compared to the PPAR null group. (*
with statistical analysis using ANOVA).
