Screening for PPAR Responsive Regulatory Modules in Cancer

Figure 3

A gene module map compiled from bioinformatics data and experimental datasets. The superimposition of the PPRE track (in green on top) on other genome-wide datasets can reveal promising PPRE-containing binding modules for targeted therapy via PPAR activation. In this imaginary setting, transcription factor 1 (in blue) is known to be one main regulator of the hypothetical gene X and this regulation is altered in cancer. Transcription factor 2 (in yellow) synergistically activates gene X, but is lost in cancer cells. Chromatin immunoprecipitation data comparing normal and cancer binding profiles for this transcription factor reveal two main regulatory modules under normal conditions and a weaker binding in cancer samples due to loss of transcription factor 2. A colocalizing PPRE in module 2 could enable PPARs to replace transcription factor 2 in this module and to restore strong activation of this gene.