Rosiglitazone Inhibits Adrenocortical Cancer Cell Proliferation by Interfering with the IGF-IR Intracellular Signaling

Figure 3

IGF-induced cell proliferation is differently affected by RGZ in SW13 and H295R cell lines. SW13 (a)–(e) and H295R (f)–(h) proliferation was evaluated by MTS assay following stimulation with increasing doses of IGF-I in the presence or absence of 20 𝜇M RGZ for the indicated times. Mean ± SE percentage of OD over respective control. Statistical significance: ^∗𝑃<0.05, §𝑃<0.005, #𝑃<0.001 versus the corresponding points with RGZ, n = 6. Total number/well of SW13 (i) and H295R (j) cells treated with 10 nM IGF-I and 20 𝜇M RGZ for 2 and 4 days, respectively, was evaluated by cell counting under optical microscope. Data are expressed as mean ± SE of cell number evaluated in triplicate in three independent experiments. ^°𝑃<.01, #𝑃<.001 versus Ctrl; ^∗𝑃<.001 versus IGF-I.

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