Rosiglitazone Inhibits Adrenocortical Cancer Cell Proliferation by Interfering with the IGF-IR Intracellular Signaling
Figure 4
RGZ blunts IGF-I stimulation of
Akt phosphorylation/activation. Western blot analysis of SW13 (a) and H295R
(b) cell lysates following 15-minutestimulation with 10 nM IGF-I and 20 M RGZ, reveals an increased
phosphorylation of Akt in Ser473 following IGF-I, which is reverted by RGZ.
Lane protein normalization for Akt is shown in the middle panels. Molecular
weight markers are indicated. Mean SE of phospho-Akt band intensity over
Ctrl is shown in 4 independent experiments for SW13 (a) and H295R (b) cells (lower
panels). Statistical
significance: versus Ctrl or IGF + RGZ.
