Rosiglitazone Inhibits Adrenocortical Cancer Cell Proliferation by Interfering with the IGF-IR Intracellular Signaling
Figure 6
RGZ dampens IGF-I stimulated
ERK1/2 phosphorylation. Western blot analysis of SW13 (a) and H295R (b)
cell lysates following 15-minutes stimulation with 10 nM IGF-I and 20 M RGZ, reveals an increased
phosphorylation of ERK1/2 following IGF-I, which is reverted by RGZ. Lane
protein normalization for ERK1/2 is shown in the middle panels. Molecular
weight markers are indicated. Mean SE of phospho-ERK1/2 band intensity
over respective Ctrl is shown in 3 independent experiments for SW13 (a) and
H295R (b) cells (lower panels).
versus respective Ctrl; ° and # versus IGF-I.
