Differences in Transcriptional Activation by the Two Allelic (L162V Polymorphic) Variants of PPAR𝛼 after Omega-3 Fatty Acids Treatment

Figure 1

Transcriptional activity by L162-PPAR𝛼 and V162-PPAR𝛼 in HepG2 cells supplemented with EPA, DHA, and mixtures of EPA:DHA. The DR1-PPRE-TKpGL3 reporter construct (100 ng) was cotransfected with the pRL-NULL plasmid (30 ng) in HepG2 cells in presence of 10 ng pSG5-hPPAR𝛼 wild-type (black bars) or mutated (white bars) and pSG5-mRXR𝛼 (10 ng) plasmids. Cells were subsequently treated or not with ciprofibrate (250 𝜇M) or varying concentrations and mixtures of EPA and/or DHA for 24 hours. Values were normalized to internal Renilla luciferase activity as described in materials and methods and expressed as fold-induction relative to the control (TK-pGL3) set at 1. Values are representative of 2 independent experiments realized in triplicates.