Fasting Upregulates PPAR Target Genes in Brain and Influences Pituitary Hormone Expression in a PPAR Dependent Manner
Figure 3
Effect of fasting on mRNA concentrations of acyl-CoA oxidase (ACO) and mitochondrial 3-hydroxy-3-methylglutaryl-(mHMG)-CoA synthase in pituitary gland and liver of wild-type () and PPARĪ± knockout mice (). Mice of both genotypes were either fasted for 48ā€‰hours (control group) or fed a standard rodent diet ad libitum for 48ā€‰hours (fed group). Livers and pituitary glands of mice were excised, pituitary glands from two animals were pooled, total RNA was extracted, and mRNA abundances were determined by real-time detection RT-PCR analysis using -actin for normalization. Bars represent means SD (liver: ; pituitary: ). Means without a common letter differ, . Significant effects () from two-way ANOVA: mHMG-CoA synthase (pituitary): genotype, fasting, fasting genotype (); ACO (liver): genotype; mHMG-CoA synthase (liver): fasting, genotype, fasting genotype.