Research Article

Molecular Characterization of the Tumor Suppressor Candidate 5 Gene: Regulation by PPAR and Identification of TUSC5 Coding Variants in Lean and Obese Humans

Figure 2

Temporal Changes in Histone Modifications and PPAR Binding at Putative DR1 Sites in the Promoter Region of the Murine TUSC5 Gene During 3T3-L1 Adipocyte Differentiation. ChIP studies were performed in preadipocytes and maturing adipocytes at days 4 and 8 days postdifferentiation initiation, employing anti-acetylated histone H3 (K9), anti-acetylated histone H4 (pan), or anti-PPAR antibodies and sequence-specific primers for putative DR1 sites (PPAR-response elements) located at promoter regions −8.4 and −1.1 kb relative to the murine TUSC5 start codon. Shown are images representative of 3 independent experiments.
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