Molecular Characterization of the Tumor Suppressor Candidate 5 Gene: Regulation by PPAR𝛾 and Identification of TUSC5 Coding Variants in Lean and Obese Humans

Figure 6

Locations of Single-Nucleotide Polymorphisms (SNPs) Impacting the Coding Region of Human TUSC5. A number of SNPs were detected in the course of resequencing the TUSC5 open reading frame of >700 human subjects (see Table 2), with 5 SNPs encoding amino acid changes (see diamonds; residue changes are noted). Notably, the I106T shift is within the CD225 domain of Tusc5 (domain bordered by arrows), and the P15S change is at a site highly-conserved across species. The alignment tool in VectorNTI (Invitrogen) was used to compare known and putative Tusc5 GenBank sequences from two human entries (BAC43751 [HUMAN-A] and NP_758955 [HUMAN-B]), chimpanzees (XP_001153397), mice (NP_808377), rats (NP_001034252), dogs (XP_548306), and cattle (XP_605998).