PRIC295, a Nuclear Receptor Coactivator, Identified from PPAR-Interacting Cofactor Complex
Figure 1
PRIC295 in ciprofibrate bound protein complex. (a) Ciprofibrate-binding protein complex isolated from rat liver nuclear extracts analyzed on a 3%–8% Tris-acetate silver stained gel. Lane 1 marker; lane 2 BP, binding proteins. Selected high molecular weight bands (arrows) were excised from the gel and digested with trypsin. MALDI-TOF analysis was conducted on the marked bands yielding peptide-matching protein identities, in descending order from highest molecular weight to lowest, of PRIC295, PRIC285, CBP, PRIC250, and TRIP230. (b) Mass spectrometric and limited sequence analysis data of proteins identified by MALDI-TOF with their associated peptide fragment sequences, m/z ratios and corresponding accession numbers.
