PRIC295, a Nuclear Receptor Coactivator, Identified from PPAR-Interacting Cofactor Complex
Figure 7
PRIC295-interacting proteins. (a) PRIC295-interacting proteins were isolated using GST-Δ (F2) (pGEX-Δ) to pull down interacting proteins from rat liver nuclear extract. Selected bands (lane 2-BP; arrows) were excised from the 4–20% Tris-HCl gel and digested with trypsin to release peptide fragments. (b) Peptide fragments derived from excised bands were subjected to MALDI-TOF analysis. Proteins matching the identities with peptide fragments are shown with some matching peptide sequences, m/z ratios, and accession numbers. In (a), immunoblotting of ciprofibrate-binding protein complex reveals the presence of PPAR (lane 3), Med24/TRAP100 (lane 4), and Med1 (lane 5).
