PRIC295, a Nuclear Receptor Coactivator, Identified from PPAR-Interacting Cofactor Complex
Figure 8
Intracellular localization of PRIC295. (a) Immunofluorescence microscopy to visualize intracellular localization of PRIC295-3xFLAG and PPAR in HeLa cancer cells after transfection with pcDNA-PRIC295-3xFLAG and pCMX-PPAR. Anti-FLAG M2 monoclonal antibody was used for PRIC295-3xFLAG (green color), and anti-PPAR antibody was used to stain PPAR (red color). DAPI nuclear stain is for the visualization of cells in the field. Merged image of PRIC295-3xFLAG (green) and PPAR (red) reveals yellow coloration of nuclei that coexpress both proteins. The presence of a green and red cell in the merged panel is an indication that these cells are not likely transfected with both plasmids. (b) Immunoprecipitation data showing purified PRIC295-3xFLAG protein and coimmunoprecipitated proteins PPAR, Med24/TRAP100, and Med1. Cells were transfected with pcDNA-PRIC295-3xFLAG only.
