PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues

Figure 1

Novel insight into PPARG world trough new approaches. In the presence of ligands (upper), PPAR𝛾 binds to its PPRE as heterodimer with RXR to activate or repress target genes’ expression. The figure summarizes novel molecular mechanisms of PPAR𝛾 obtained through ChIP-seq. PPAR𝛾- and RXR-binding sites detected by ChIP-seq reveal different spatial and temporal activation of distinct metabolic pathways and changes in RXR dimer composition during adipogenesis (right panel, study from [28]). 𝑃𝑃𝐴𝑅𝛾 in Adipocytes and Macrophages: tissue-specific regulatory regions employ cell-type-specific coregulators, C/EBP𝛽 in adipocytes and PU.1 in macrophages (lower panel; ChIP-Seq study from [31]).