PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues

Figure 2

Innovative approaches by using next generation sequencing technologies. Next generation sequencing can be applied to many scientific contexts: targeted resequencing for the identification of disease-causing nucleotide variations for both coding and unexplored regulatory regions of genes (CNV-Seq and DNA-Seq); ChIP-Seq, for DNA-protein interaction studies coupling chromatin immuno-precipitation (ChIP) and massively parallel sequencing; RNA-Seq, for whole transcriptome studies, including expression levels of known and yet unknown transcripts (both coding and non-coding), differential splicing, allele-specific expression, RNA editing, and fusion transcripts (see review [121]).