Peroxisome Proliferator-Activated Receptors Alpha, Beta, and Gamma mRNA and Protein Expression in Human Fetal Tissues

Figure 6

Intestine. (a) The expression of PPAR$\alpha \text{,}$$\beta$, and $\gamma$ mRNA is shown across the fetal age range. Log plot of mean $\pm$ SEM Ct is normalized to $\beta$-2-microglobulin (B2M). (b) The fetal expression of PPAR$\alpha \text{,}$$\beta$, and $\gamma$ is shown relative to expression in adult small intestine and colon (filled circles show the 2 replicates of small intestine above and 2 replicates of colon below the line indicating the mean of the combined tissue values). Each open symbol represents the mean Ct value of 2 replicates for each fetal sample (overall mean is shown as a horizontal line). (c) PPAR$\alpha \text{,}$$\beta$, and $\gamma$ protein expression is shown across the fetal age range. Western blot density is normalized to glyceraldehyde-3-phophate dehydrogenase (GAPDH). Up arrowhead indicates PPAR$\alpha$, down arrowhead PPAR$\beta$, and diamond PPAR$\gamma$. If only one sample was available for a particular age, then an error term could not be calculated and no SEM bar is shown. Regression analysis evaluated change with age. Dashed lines in graphs of C are the 95% confidence interval.